IDH1 CLIA Kit
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- 抗原 See all IDH1 CLIA Kits
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 7.81 ng/mL - 500 ng/mL
- 最低检测浓度
- 7.81 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Isocitrate Dehydrogenase 1, Soluble (IDH1). No significant cross-reactivity or interference between Isocitrate Dehydrogenase 1, Soluble (IDH1) and analogues was observed.
- 灵敏度
- 0.28 ng/mL
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Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) ELISA Kit
IDH1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) ELISA KitIDH1 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Isocitrate Dehydrogenase 1, Soluble (IDH1) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all IDH1 CLIA Kits
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
- 别名
- Isocitrate Dehydrogenase 1, Soluble (IDH1 产品)
- 别名
- IDCD CLIA Kit, IDH CLIA Kit, IDP CLIA Kit, IDPC CLIA Kit, PICD CLIA Kit, NADP-CICDH CLIA Kit, AI314845 CLIA Kit, AI788952 CLIA Kit, E030024J03Rik CLIA Kit, Id-1 CLIA Kit, Idh-1 CLIA Kit, Idpc CLIA Kit, cb876 CLIA Kit, fm90e09 CLIA Kit, im:7143416 CLIA Kit, wu:fm90e09 CLIA Kit, F23E12.180 CLIA Kit, F23E12_180 CLIA Kit, IDH-I CLIA Kit, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 CLIA Kit, isocitrate dehydrogenase 1 CLIA Kit, isocitrate dehydrogenase I CLIA Kit, isocitrate dehydrogenase (NADP(+)) 1, cytosolic CLIA Kit, isocitrate dehydrogenase 1 (NADP+), soluble CLIA Kit, isocitrate dehydrogenase 1 (NADP+) L homeolog CLIA Kit, isocitrate dehydrogenase 1 CLIA Kit, IDH1 CLIA Kit, Idh1 CLIA Kit, idh1.L CLIA Kit, idh1 CLIA Kit
- 背景
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Gene Name: Isocitrate Dehydrogenase 1, Soluble
Gene Aliases: PICD, IDP, Oxalosuccinate decarboxylase, NADP(+)-specific ICDH, Cytosolic NADP-isocitrate dehydrogenase
- 基因ID
- 3417
- UniProt
- O75874
- 途径
- Warburg Effect
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