IL-10RA ELISA 试剂盒
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- 抗原 See all IL-10RA (IL10RA) ELISA试剂盒
- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Interleukin 10 Receptor Alpha (IL10Ra). No significant cross-reactivity or interference between Interleukin 10 Receptor Alpha (IL10Ra) and analogues was observed.
- 灵敏度
- 11.3 pg/mL
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Interleukin 10 Receptor, alpha (IL10RA) ELISA Kit
IL10RA 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
Interleukin 10 Receptor, alpha (IL10RA) ELISA KitIL10RA 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Interleukin 10 Receptor, alpha (IL10RA) ELISA KitIL10RA 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Interleukin 10 Receptor, alpha (IL10RA) ELISA KitIL10RA 适用: 人 Colorimetric Sandwich ELISA 2.5-500 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 Receptor Alpha (IL10Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 Receptor Alpha (IL10Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 Receptor Alpha (IL10Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 10 Receptor Alpha (IL10Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 Receptor Alpha (IL10Ra) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 Receptor Alpha (IL10Ra) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all IL-10RA (IL10RA) ELISA试剂盒
- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
- 别名
- Interleukin 10 Receptor Alpha (IL10RA 产品)
- 别名
- IL10R1 ELISA Kit, IL10RA ELISA Kit, CD210 ELISA Kit, CD210a ELISA Kit, CDW210A ELISA Kit, HIL-10R ELISA Kit, IL-10R1 ELISA Kit, IL10R ELISA Kit, AW553859 ELISA Kit, CDw210 ELISA Kit, CDw210a ELISA Kit, Il10r ELISA Kit, mIL-10R ELISA Kit, interleukin 10 receptor subunit alpha ELISA Kit, interleukin 10 receptor, alpha ELISA Kit, IL10RA ELISA Kit, Il10ra ELISA Kit
- 背景
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Gene Name: Interleukin 10 Receptor Alpha
Gene Aliases: CDW210A, CD210A, IL10R-A, IL10-RA, CD210-A, HIL-10R, IL-10R1, IL10R, Cluster Of Differentiation W210A
- 基因ID
- 3587
- UniProt
- Q13651
- 途径
- Growth Factor Binding
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