IRAK4 ELISA 试剂盒
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- 抗原 See all IRAK4 ELISA试剂盒
- IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Associated Kinase 4 (IRAK4). No significant cross-reactivity or interference between Interleukin 1 Receptor Associated Kinase 4 (IRAK4) and analogues was observed.
- 灵敏度
- 0.64 ng/mL
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Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) ELISA Kit
IRAK4 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) ELISA KitIRAK4 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) ELISA KitIRAK4 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 4 (IRAK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Associated Kinase 4 (IRAK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 4 (IRAK4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 4 (IRAK4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all IRAK4 ELISA试剂盒
- IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))
- 别名
- Interleukin 1 Receptor Associated Kinase 4 (IRAK4 产品)
- 别名
- zgc:55553 ELISA Kit, IRAK4 ELISA Kit, ipd1 ELISA Kit, irak-4 ELISA Kit, ny-ran-64 ELISA Kit, ny-ren-64 ELISA Kit, ren64 ELISA Kit, irak4 ELISA Kit, IPD1 ELISA Kit, IRAK-4 ELISA Kit, NY-REN-64 ELISA Kit, REN64 ELISA Kit, 8430405M07Rik ELISA Kit, 9330209D03Rik ELISA Kit, interleukin 1 receptor associated kinase 4 ELISA Kit, interleukin-1 receptor-associated kinase 4 ELISA Kit, IRAK4 ELISA Kit, irak4 ELISA Kit, Irak4 ELISA Kit
- 背景
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Gene Name: Interleukin 1 Receptor Associated Kinase 4
Gene Aliases: NY-REN-64
- 基因ID
- 51135
- UniProt
- Q9NWZ3
- 途径
- NF-kappaB Signaling, TLR signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades
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