IGF2R ELISA 试剂盒
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- 抗原 See all IGF2R ELISA试剂盒
- IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.625 ng/mL - 40 ng/mL
- 最低检测浓度
- 0.625 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 2 Receptor (IGF2R). No significant cross-reactivity or interference between Insulin Like Growth Factor 2 Receptor (IGF2R) and analogues was observed.
- 灵敏度
- 0.221 ng/mL
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Insulin-Like Growth Factor 2 Receptor (IGF2R) ELISA Kit
IGF2R 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 2 Receptor (IGF2R) ELISA KitIGF2R 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Insulin-Like Growth Factor 2 Receptor (IGF2R) ELISA KitIGF2R 适用: 人 AA 1510-2108 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Insulin-Like Growth Factor 2 Receptor (IGF2R) ELISA KitIGF2R 适用: 人 Colorimetric Sandwich ELISA 0.27-200 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 Receptor (IGF2R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 Receptor (IGF2R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 2 Receptor (IGF2R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 2 Receptor (IGF2R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 2 Receptor (IGF2R) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 2 Receptor (IGF2R) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all IGF2R ELISA试剂盒
- IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))
- 别名
- Insulin Like Growth Factor 2 Receptor (IGF2R 产品)
- 别名
- CD222 ELISA Kit, CIMPR ELISA Kit, M6P-R ELISA Kit, MPR1 ELISA Kit, MPRI ELISA Kit, AI661837 ELISA Kit, CI-MPR ELISA Kit, M6P/IGF2R ELISA Kit, Mpr300 ELISA Kit, mpri ELISA Kit, cd222 ELISA Kit, cimpr ELISA Kit, m6p-r ELISA Kit, insulin like growth factor 2 receptor ELISA Kit, insulin-like growth factor 2 receptor ELISA Kit, IGF2R ELISA Kit, Igf2r ELISA Kit, igf2r ELISA Kit
- 背景
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Gene Name: Insulin Like Growth Factor 2 Receptor
Gene Aliases: CD222, IGF2-R, CIMPR, M6P-R, MPRI, Cation-Independent Mannose-6 Phosphate Receptor, M6P/IGF2 receptor, 300 kDa mannose 6-phosphate receptor
- 基因ID
- 3482
- UniProt
- P11717
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