IGF2 ELISA 试剂盒
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- 抗原 See all IGF2 ELISA试剂盒
- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 2 (IGF2). No significant cross-reactivity or interference between Insulin Like Growth Factor 2 (IGF2) and analogues was observed.
- 灵敏度
- 6.8 pg/mL
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- Discover our top product IGF2 ELISA Kit
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 (IGF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 (IGF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 2 (IGF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 2 (IGF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 2 (IGF2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 2 (IGF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all IGF2 ELISA试剂盒
- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
- 别名
- Insulin Like Growth Factor 2 (IGF2 产品)
- 别名
- C11orf43 ELISA Kit, IGF-II ELISA Kit, PP9974 ELISA Kit, LOC100136550 ELISA Kit, igf2 ELISA Kit, igf2b ELISA Kit, wu:fc26e03 ELISA Kit, zgc:91781 ELISA Kit, igf-2 ELISA Kit, insigf ELISA Kit, pp9974 ELISA Kit, AL033362 ELISA Kit, Igf-2 ELISA Kit, Igf-II ELISA Kit, M6pr ELISA Kit, Mpr ELISA Kit, Peg2 ELISA Kit, IGFII ELISA Kit, RNIGF2 ELISA Kit, IGF-II-B ELISA Kit, igf2-a ELISA Kit, igf2-b ELISA Kit, insulin like growth factor 2 ELISA Kit, insulin-like growth factor II ELISA Kit, insulin-like growth factor 2a ELISA Kit, insulin like growth factor 2 S homeolog ELISA Kit, insulin-like growth factor 2 ELISA Kit, insulin-like growth factor 2 (somatomedin A) ELISA Kit, insulin like growth factor 2 L homeolog ELISA Kit, IGF2 ELISA Kit, LOC100136550 ELISA Kit, igf2a ELISA Kit, igf2.S ELISA Kit, Igf2 ELISA Kit, igf2.L ELISA Kit
- 背景
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Gene Name: Insulin Like Growth Factor 2
Gene Aliases: INSIGF, IGF-II, Somatomedin A, Preptin, Insulin-like growth factor II Ala-25 Del
- 基因ID
- 16002
- 途径
- Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
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