IDE ELISA 试剂盒
IDE
适用: 大鼠
Colorimetric
Sandwich ELISA
0.312 ng/mL - 20 ng/mL
Cell Lysate, Tissue Homogenate
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- 抗原 See all IDE ELISA试剂盒
- IDE (Insulin-Degrading Enzyme (IDE))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Insulin Degrading Enzyme (IDE). No significant cross-reactivity or interference between Insulin Degrading Enzyme (IDE) and analogues was observed.
- 灵敏度
- 0.125 ng/mL
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Degrading Enzyme (IDE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Degrading Enzyme (IDE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Degrading Enzyme (IDE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Degrading Enzyme (IDE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Degrading Enzyme (IDE) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Degrading Enzyme (IDE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all IDE ELISA试剂盒
- IDE (Insulin-Degrading Enzyme (IDE))
- 别名
- Insulin Degrading Enzyme (IDE 产品)
- 别名
- zgc:162603 ELISA Kit, IDE ELISA Kit, 1300012G03Rik ELISA Kit, 4833415K22Rik ELISA Kit, AA675336 ELISA Kit, AI507533 ELISA Kit, INSDEGM ELISA Kit, INSULYSIN ELISA Kit, insulin degrading enzyme ELISA Kit, insulin-degrading enzyme ELISA Kit, metallopeptidase ELISA Kit, IDE ELISA Kit, ide ELISA Kit, LOC591315 ELISA Kit, CC1G_01115 ELISA Kit, Bm1_26615 ELISA Kit, PTRG_10875 ELISA Kit, SJAG_01430 ELISA Kit, EBI_21700 ELISA Kit, VDBG_06702 ELISA Kit, LOAG_08082 ELISA Kit, PGTG_04982 ELISA Kit, Tsp_00424 ELISA Kit, Ide ELISA Kit
- 背景
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Gene Name: Insulin Degrading Enzyme
Gene Aliases: Insulysin, Insulin Protease, Abeta-degrading protease, Insulinase
- 途径
- SARS-CoV-2 Protein Interactome
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