HMBS ELISA 试剂盒
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- 抗原 See all HMBS ELISA试剂盒
- HMBS (Hydroxymethylbilane Synthase (HMBS))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Hydroxymethylbilane Synthase (HMBS). No significant cross-reactivity or interference between Hydroxymethylbilane Synthase (HMBS) and analogues was observed.
- 灵敏度
- 0.26 ng/mL
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Hydroxymethylbilane Synthase (HMBS) ELISA Kit
HMBS 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hydroxymethylbilane Synthase (HMBS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hydroxymethylbilane Synthase (HMBS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hydroxymethylbilane Synthase (HMBS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydroxymethylbilane Synthase (HMBS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hydroxymethylbilane Synthase (HMBS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hydroxymethylbilane Synthase (HMBS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all HMBS ELISA试剂盒
- HMBS (Hydroxymethylbilane Synthase (HMBS))
- 别名
- Hydroxymethylbilane Synthase (HMBS 产品)
- 别名
- PBG-D ELISA Kit, PBGD ELISA Kit, PORC ELISA Kit, UPS ELISA Kit, URO-S ELISA Kit, hemC ELISA Kit, pbg-d ELISA Kit, pbgd ELISA Kit, ups ELISA Kit, SPAC806.01 ELISA Kit, DDBDRAFT_0186148 ELISA Kit, DDBDRAFT_0231417 ELISA Kit, DDB_0186148 ELISA Kit, DDB_0231417 ELISA Kit, Hmbs ELISA Kit, NV50236 ELISA Kit, T25658 ELISA Kit, Ups ELISA Kit, Uros1 ELISA Kit, hmbs ELISA Kit, zgc:64128 ELISA Kit, hmbsl ELISA Kit, id:ibd5004 ELISA Kit, im:7140060 ELISA Kit, zgc:110690 ELISA Kit, hydroxymethylbilane synthase ELISA Kit, hydroxymethylbilane synthase S homeolog ELISA Kit, hydroxymethylbilane synthase L homeolog ELISA Kit, hydroxymethylbilane synthase (predicted) ELISA Kit, Hydroxymethylbilane synthase ELISA Kit, hydroxymethylbilane synthase a ELISA Kit, hydroxymethylbilane synthase, b ELISA Kit, HMBS ELISA Kit, Hmbs ELISA Kit, hmbs ELISA Kit, hmbs.2.S ELISA Kit, hmbs.L ELISA Kit, CNC02250 ELISA Kit, hem3 ELISA Kit, hemC ELISA Kit, Trad_0335 ELISA Kit, Plabr_2100 ELISA Kit, Sgly_3113 ELISA Kit, SMLT_RS19660 ELISA Kit, PMI_RS16580 ELISA Kit, hmbsa ELISA Kit, hmbsb ELISA Kit
- 背景
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Gene Name: Hydroxymethylbilane Synthase
Gene Aliases: PBG-D, PBGD, UPS, Hydroxymethylbilane Synthase, Uroporphyrinogen I Synthase, Porphobilinogen deaminase, Pre-uroporphyrinogen synthase
- 基因ID
- 3145
- UniProt
- P08397
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