BAT3 ELISA 试剂盒
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- 抗原 See all BAT3 ELISA试剂盒
- BAT3 (Large Proline-Rich Protein BAT3 (BAT3))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of HLA-B Associated Transcript 3 (BAT3). No significant cross-reactivity or interference between HLA-B Associated Transcript 3 (BAT3) and analogues was observed.
- 灵敏度
- 0.053 ng/mL
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Large Proline-Rich Protein BAT3 (BAT3) ELISA Kit
BAT3 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Large Proline-Rich Protein BAT3 (BAT3) ELISA KitBAT3 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLA-B Associated Transcript 3 (BAT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HLA-B Associated Transcript 3 (BAT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HLA-B Associated Transcript 3 (BAT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HLA-B Associated Transcript 3 (BAT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HLA-B Associated Transcript 3 (BAT3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HLA-B Associated Transcript 3 (BAT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all BAT3 ELISA试剂盒
- BAT3 (Large Proline-Rich Protein BAT3 (BAT3))
- 别名
- HLA-B Associated Transcript 3 (BAT3 产品)
- 别名
- ARABIDOPSIS THALIANA BCL-2-ASSOCIATED ATHANOGENE 6 ELISA Kit, ATBAG6 ELISA Kit, BCL-2-associated athanogene 6 ELISA Kit, T3F17.11 ELISA Kit, 2410045D21Rik ELISA Kit, AA408914 ELISA Kit, BAG-6 ELISA Kit, Bat3 ELISA Kit, D17H6S52E ELISA Kit, G3 ELISA Kit, Scythe ELISA Kit, BAT3 ELISA Kit, D6S52E ELISA Kit, BCL-2-associated athanogene 6 ELISA Kit, BCL2-associated athanogene 6 ELISA Kit, BCL2 associated athanogene 6 ELISA Kit, BAG6 ELISA Kit, Bag6 ELISA Kit
- 背景
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Gene Name: HLA-B Associated Transcript 3
Gene Aliases: BAG6, Protein G3, Protein Scythe, BCL2-associated athanogene 6, BAG family molecular chaperone regulator 6, Large proline-rich protein BAG6
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