Histone H4 ELISA 试剂盒
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- 抗原 See all Histone H4 ELISA试剂盒
- Histone H4
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适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Histone H4 (H4). No significant cross-reactivity or interference between Histone H4 (H4) and analogues was observed.
- 灵敏度
- 0.056 ng/mL
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Histone H4 ELISA Kit
适用: 人, 小鼠, 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Histone H4 ELISA Kit适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H4 (H4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone H4 (H4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone H4 (H4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone H4 (H4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone H4 (H4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone H4 (H4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Histone H4 ELISA试剂盒
- Histone H4
- Abstract
- Histone H4 产品
- 别名
- hist1h4l ELISA Kit, H1ft ELISA Kit, Hist4 ELISA Kit, Hist4h4 ELISA Kit, H4 ELISA Kit, X04652 ELISA Kit, FO108 ELISA Kit, H4/n ELISA Kit, H4F2 ELISA Kit, H4FN ELISA Kit, HIST2H4 ELISA Kit, H4C13 ELISA Kit, H4C7 ELISA Kit, F8N16.2 ELISA Kit, F8N16_2 ELISA Kit, histone H4 ELISA Kit, BcDNA:RH52884 ELISA Kit, CG3379 ELISA Kit, Dmel\CG3379 ELISA Kit, H4r ELISA Kit, His4-88CD ELISA Kit, His4R ELISA Kit, histone cluster 1, H4a L homeolog ELISA Kit, histone cluster 1 H4 family member B ELISA Kit, histone cluster 2, H4 ELISA Kit, histone cluster 2 H4 family member a ELISA Kit, histone H4 ELISA Kit, Histone H4 replacement ELISA Kit, Histone H4 ELISA Kit, hist1h4a.L ELISA Kit, Hist1h4b ELISA Kit, Hist2h4 ELISA Kit, HIST2H4A ELISA Kit, H4 ELISA Kit, H4C14 ELISA Kit, HIS4 ELISA Kit, HIST2H4 ELISA Kit, His4r ELISA Kit, his-38 ELISA Kit
- 背景
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Gene Name: Histone H4
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