HRG ELISA 试剂盒
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- 抗原 See all HRG ELISA试剂盒
- HRG (Histidine-Rich Glycoprotein (HRG))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.469 ng/mL - 30 ng/mL
- 最低检测浓度
- 0.469 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Histidine Rich Glycoprotein (HRG). No significant cross-reactivity or interference between Histidine Rich Glycoprotein (HRG) and analogues was observed.
- 灵敏度
- 0.21 ng/mL
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Histidine-Rich Glycoprotein (HRG) ELISA Kit
HRG 适用: 人 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Plasma, Serum
Histidine-Rich Glycoprotein (HRG) ELISA KitHRG 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma
Histidine-Rich Glycoprotein (HRG) ELISA KitHRG 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Histidine-Rich Glycoprotein (HRG) ELISA KitHRG 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Histidine-Rich Glycoprotein (HRG) ELISA KitHRG 适用: 小鼠 Colorimetric Competition ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Histidine-Rich Glycoprotein (HRG) ELISA KitHRG 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Rich Glycoprotein (HRG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Rich Glycoprotein (HRG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Rich Glycoprotein (HRG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidine Rich Glycoprotein (HRG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histidine Rich Glycoprotein (HRG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histidine Rich Glycoprotein (HRG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all HRG ELISA试剂盒
- HRG (Histidine-Rich Glycoprotein (HRG))
- 别名
- Histidine Rich Glycoprotein (HRG 产品)
- 别名
- AI265597 ELISA Kit, AW413091 ELISA Kit, D16jh2 ELISA Kit, D18020 ELISA Kit, Hprg ELISA Kit, Hrgp ELISA Kit, HPRG ELISA Kit, HRGP ELISA Kit, THPH11 ELISA Kit, HRG1 ELISA Kit, histidine rich glycoprotein ELISA Kit, histidine-rich glycoprotein ELISA Kit, HRG ELISA Kit, LOAG_12427 ELISA Kit, LOC100597044 ELISA Kit, Hrg ELISA Kit
- 背景
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Gene Name: Histidine Rich Glycoprotein
Gene Aliases: HPRG, HRGP, Histidine-Proline Rich Glycoprotein, Thrombophilia Due To Elevated HRG
- UniProt
- P04196
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