HMGA1 ELISA 试剂盒
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- 抗原 See all HMGA1 ELISA试剂盒
- HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of High Mobility Group AT Hook Protein 1 (HMGA1). No significant cross-reactivity or interference between High Mobility Group AT Hook Protein 1 (HMGA1) and analogues was observed.
- 灵敏度
- 0.124 ng/mL
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High Mobility Group AT-Hook 1 (HMGA1) ELISA Kit
HMGA1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
High Mobility Group AT-Hook 1 (HMGA1) ELISA KitHMGA1 适用: 犬 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
High Mobility Group AT-Hook 1 (HMGA1) ELISA KitHMGA1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group AT Hook Protein 1 (HMGA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group AT Hook Protein 1 (HMGA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group AT Hook Protein 1 (HMGA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Mobility Group AT Hook Protein 1 (HMGA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group AT Hook Protein 1 (HMGA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Mobility Group AT Hook Protein 1 (HMGA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all HMGA1 ELISA试剂盒
- HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))
- 别名
- High Mobility Group AT Hook Protein 1 (HMGA1 产品)
- 别名
- HMG-R ELISA Kit, HMGA1A ELISA Kit, HMGIY ELISA Kit, Hmgi ELISA Kit, Hmgiy ELISA Kit, hmga1 ELISA Kit, hmgiy ELISA Kit, wu:fb12b06 ELISA Kit, wu:fb18f11 ELISA Kit, wu:fc19f01 ELISA Kit, wu:fi38b05 ELISA Kit, zgc:85677 ELISA Kit, AL023995 ELISA Kit, Hmga1a ELISA Kit, Hmga1b ELISA Kit, Hmgy ELISA Kit, HMG-I(Y) ELISA Kit, HMG-Y ELISA Kit, HMGA1 ELISA Kit, zgc:153948 ELISA Kit, high mobility group AT-hook 1 ELISA Kit, high mobility group protein HMG-I/HMG-Y ELISA Kit, high mobility group AT-hook 1a ELISA Kit, high mobility group AT-hook 1b ELISA Kit, HMGA1 ELISA Kit, Hmga1 ELISA Kit, TSC_c24220 ELISA Kit, hmga1a ELISA Kit, LOC705105 ELISA Kit, hmga1b ELISA Kit
- 背景
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Gene Name: High Mobility Group AT Hook Protein 1
Gene Aliases: HMG-R, HMGIY, High mobility group protein A1, High mobility group protein R, High mobility group protein HMG-I/HMG-Y
- 基因ID
- 3159
- UniProt
- P17096
- 途径
- Nuclear Hormone Receptor Binding, Autophagy
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