HSPA1B ELISA 试剂盒
-
- 抗原 See all HSPA1B ELISA试剂盒
- HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))
-
适用
- Cow
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Heat Shock 70 kDa Protein 1B (HSPA1B). No significant cross-reactivity or interference between Heat Shock 70 kDa Protein 1B (HSPA1B) and analogues was observed.
- 灵敏度
- 0.53 ng/mL
- Featured
- Discover our best selling HSPA1B ELISA Kit
- Top Product
- Discover our top product HSPA1B ELISA Kit
-
Heat Shock 70kDa Protein 1B (HSPA1B) ELISA Kit
HSPA1B 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock 70kDa Protein 1B (HSPA1B) ELISA KitHSPA1B 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70 kDa Protein 1B (HSPA1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70 kDa Protein 1B (HSPA1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70 kDa Protein 1B (HSPA1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70 kDa Protein 1B (HSPA1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 1B (HSPA1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 1B (HSPA1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all HSPA1B ELISA试剂盒
- HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))
- 别名
- Heat Shock 70kDa Protein 1B (HSPA1B 产品)
- 别名
- HSP70-1B ELISA Kit, HSP70-2 ELISA Kit, APG-2 ELISA Kit, HS24/P52 ELISA Kit, HSPH2 ELISA Kit, RY ELISA Kit, hsp70 ELISA Kit, hsp70RY ELISA Kit, ARABIDOPSIS THALIANA HEAT SHOCK COGNATE PROTEIN 70-1 ELISA Kit, AT-HSC70-1 ELISA Kit, HEAT SHOCK COGNATE PROTEIN 70 ELISA Kit, HEAT SHOCK PROTEIN 70-1 ELISA Kit, HSC70 ELISA Kit, HSP70-1 ELISA Kit, T22P11.90 ELISA Kit, T22P11_90 ELISA Kit, heat shock cognate protein 70-1 ELISA Kit, Hsp70 ELISA Kit, Hsp70-1 ELISA Kit, Hsp70.1 ELISA Kit, hsp68 ELISA Kit, HSPA1A ELISA Kit, HSPA1B ELISA Kit, HSPA2 ELISA Kit, HSP70.2 ELISA Kit, Hsp70-2 ELISA Kit, Hsp72 ELISA Kit, Hspa1 ELISA Kit, Hspa1a ELISA Kit, Hspa2 ELISA Kit, heat shock protein family A (Hsp70) member 1B ELISA Kit, heat shock protein family A (Hsp70) member 4 ELISA Kit, heat shock cognate protein 70-1 ELISA Kit, heat shock protein 1B ELISA Kit, heat shock protein 70.2 ELISA Kit, heat shock 70kDa protein 1A ELISA Kit, heat shock 70kD protein 1B (mapped) ELISA Kit, HSPA1B ELISA Kit, HSPA4 ELISA Kit, HSC70-1 ELISA Kit, Hspa1b ELISA Kit, HSP70.2 ELISA Kit, HSPA1A ELISA Kit
- 背景
-
Gene Name: Heat Shock 70 kDa Protein 1B
Gene Aliases: HSP70-2, HSP-A1B, Heat Shock Protein 72
- 基因ID
- 282254
- UniProt
- Q27965
-
