GARS ELISA 试剂盒
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- 抗原 See all GARS ELISA试剂盒
- GARS (Glycyl-tRNA Synthetase (GARS))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Glycyl tRNA Synthetase (GARS). No significant cross-reactivity or interference between Glycyl tRNA Synthetase (GARS) and analogues was observed.
- 灵敏度
- 6.6 pg/mL
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Glycyl-tRNA Synthetase (GARS) ELISA Kit
GARS 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Glycyl-tRNA Synthetase (GARS) ELISA KitGARS 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Glycyl-tRNA Synthetase (GARS) ELISA KitGARS 适用: 人 Colorimetric Sandwich ELISA 0.938-60 ng/mL Cell Culture Cells, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycyl tRNA Synthetase (GARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycyl tRNA Synthetase (GARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycyl tRNA Synthetase (GARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycyl tRNA Synthetase (GARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycyl tRNA Synthetase (GARS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycyl tRNA Synthetase (GARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all GARS ELISA试剂盒
- GARS (Glycyl-tRNA Synthetase (GARS))
- 别名
- Glycyl tRNA Synthetase (GARS 产品)
- 别名
- CMT2D ELISA Kit, DSMAV ELISA Kit, GlyRS ELISA Kit, HMN5 ELISA Kit, SMAD1 ELISA Kit, Aat-gly ELISA Kit, CG6778 ELISA Kit, Dmel\\CG6778 ELISA Kit, GRS ELISA Kit, gars ELISA Kit, team ELISA Kit, RGD1559871 ELISA Kit, GENA202 ELISA Kit, Gena201 ELISA Kit, Nmf249 ELISA Kit, Sgrp23 ELISA Kit, GB13467 ELISA Kit, MGC79495 ELISA Kit, fb02f03 ELISA Kit, si:dkey-276i5.1 ELISA Kit, wu:fb02f03 ELISA Kit, NI36_08435 ELISA Kit, glycyl-tRNA synthetase ELISA Kit, Glycyl-tRNA synthetase ELISA Kit, glycine--tRNA ligase ELISA Kit, glycyl-tRNA synthetase L homeolog ELISA Kit, Glycine--tRNA ligase ELISA Kit, GARS ELISA Kit, GlyRS ELISA Kit, Gars ELISA Kit, LOC408392 ELISA Kit, gars.L ELISA Kit, gars ELISA Kit, LOC692991 ELISA Kit, glyQ ELISA Kit, TTHA0543 ELISA Kit, NI36_RS08405 ELISA Kit
- 背景
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Gene Name: Glycyl tRNA Synthetase
Gene Aliases: CMT2D, CMT2-D, DSMAV, GlyRS, SMAD1, EJ, Charcot-Marie-Tooth Neuropathy 2D, Glycine tRNA Ligase, Diadenosine tetraphosphate synthetase, AP-4-A synthetase
- 基因ID
- 2617
- UniProt
- P41250
- 途径
- Ribonucleoside Biosynthetic Process
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