GLDC CLIA Kit
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- 抗原 See all GLDC CLIA Kits
- GLDC (Glycine Dehydrogenase (GLDC))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 156.25 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Glycine Dehydrogenase (GLDC). No significant cross-reactivity or interference between Glycine Dehydrogenase (GLDC) and analogues was observed.
- 灵敏度
- 4.7 pg/mL
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Glycine Dehydrogenase (GLDC) ELISA Kit
GLDC 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Glycine Dehydrogenase (GLDC) ELISA KitGLDC 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glycine Dehydrogenase (GLDC) ELISA KitGLDC 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Glycine Dehydrogenase (GLDC) ELISA KitGLDC 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase (GLDC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Glycine Dehydrogenase (GLDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glycine Dehydrogenase (GLDC) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all GLDC CLIA Kits
- GLDC (Glycine Dehydrogenase (GLDC))
- 别名
- Glycine Dehydrogenase (GLDC 产品)
- 别名
- PSPTO1276 CLIA Kit, Tb07.43M14.350 CLIA Kit, DDBDRAFT_0219205 CLIA Kit, DDBDRAFT_0231130 CLIA Kit, DDB_0219205 CLIA Kit, DDB_0231130 CLIA Kit, D030049L12Rik CLIA Kit, D19Wsu57e CLIA Kit, GCE CLIA Kit, GCSP CLIA Kit, HYGN1 CLIA Kit, fb23b05 CLIA Kit, wu:fb23b05 CLIA Kit, zgc:66198 CLIA Kit, gldc CLIA Kit, glycine dehydrogenase (decarboxylating) GcvP CLIA Kit, glycine dehydrogenase CLIA Kit, glycine cleavage system P-protein CLIA Kit, glycine decarboxylase CLIA Kit, glycine dehydrogenase (decarboxylating) CLIA Kit, glycine decarboxylase S homeolog CLIA Kit, gcvP CLIA Kit, Tb927.7.1910 CLIA Kit, Gldc CLIA Kit, GLDC CLIA Kit, gldc CLIA Kit, gldc.S CLIA Kit
- 背景
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Gene Name: Glycine Dehydrogenase
Gene Aliases: GCSP, NKH, Decarboxylating, Glycine Decarboxylase, Glycine Cleavage System Protein P, Glycine dehydrogenase (aminomethyl-transferring)
- 基因ID
- 2731
- UniProt
- P23378
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