GRIN2B ELISA 试剂盒
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- 抗原 See all GRIN2B ELISA试剂盒
- GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B). No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) and analogues was observed.
- 灵敏度
- 0.117 ng/mL
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Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) ELISA Kit
GRIN2B 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) ELISA KitGRIN2B 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) ELISA KitGRIN2B 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) ELISA KitGRIN2B 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) ELISA KitGRIN2B 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all GRIN2B ELISA试剂盒
- GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))
- 别名
- Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B 产品)
- 别名
- NMDAR2B ELISA Kit, GluN2B ELISA Kit, MRD6 ELISA Kit, NR2B ELISA Kit, hNR3 ELISA Kit, nmdar2b ELISA Kit, nr2b ELISA Kit, AW490526 ELISA Kit, Nmdar2b ELISA Kit, glutamate ionotropic receptor NMDA type subunit 2B ELISA Kit, glutamate receptor, ionotropic, N-methyl D-aspartate 2B L homeolog ELISA Kit, glutamate receptor, ionotropic, NMDA2B (epsilon 2) ELISA Kit, GRIN2B ELISA Kit, Grin2b ELISA Kit, grin2b.L ELISA Kit
- 背景
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Gene Name: Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B
Gene Aliases: NMDAR2B, NR2B, hNR3, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3
- 基因ID
- 2904
- UniProt
- Q13224
- 途径
- Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
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