GFRA1 ELISA 试剂盒
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- 抗原 See all GFRA1 ELISA试剂盒
- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). No significant cross-reactivity or interference between Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) and analogues was observed.
- 灵敏度
- 0.119 ng/mL
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GDNF Family Receptor alpha 1 (GFRA1) ELISA Kit
GFRA1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
GDNF Family Receptor alpha 1 (GFRA1) ELISA KitGFRA1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
GDNF Family Receptor alpha 1 (GFRA1) ELISA KitGFRA1 适用: Pig Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all GFRA1 ELISA试剂盒
- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
- 别名
- Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRA1 产品)
- 别名
- gdnfr ELISA Kit, ret1l ELISA Kit, retl1 ELISA Kit, trnr1 ELISA Kit, gdnfra ELISA Kit, MGC122616 ELISA Kit, gfr-alpha-1 ELISA Kit, GDNFR ELISA Kit, GDNFRA ELISA Kit, GFR-ALPHA-1 ELISA Kit, RET1L ELISA Kit, RETL1 ELISA Kit, TRNR1 ELISA Kit, GDNFRALPHA ELISA Kit, GFRA1 ELISA Kit, AU042498 ELISA Kit, gfralpha1a ELISA Kit, zgc:110129 ELISA Kit, GDNF family receptor alpha 1 ELISA Kit, glial cell line derived neurotrophic factor family receptor alpha 1 ELISA Kit, gdnf family receptor alpha 1a ELISA Kit, gfra1 ELISA Kit, GFRA1 ELISA Kit, Gfra1 ELISA Kit, gfra1a ELISA Kit
- 背景
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Gene Name: Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1
Gene Aliases: GDNFR, GDNFRA, GFR-ALPHA-1, RET1L, RETL1, TRNR1, GDNF family receptor alpha-1, TGF-beta-related neurotrophic factor receptor 1
- 基因ID
- 2674
- UniProt
- P56159
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