FYN ELISA 试剂盒
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- 抗原 See all FYN ELISA试剂盒
- FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of FYN Oncogene Related To SRC/FGR/YES (FYN). No significant cross-reactivity or interference between FYN Oncogene Related To SRC/FGR/YES (FYN) and analogues was observed.
- 灵敏度
- 0.06 ng/mL
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FYN Oncogene Related To SRC, FGR, YES (FYN) ELISA Kit
FYN 适用: 人 pTyr530 Colorimetric Sandwich ELISA Cell Culture Lysate
FYN Oncogene Related To SRC, FGR, YES (FYN) ELISA KitFYN 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
FYN Oncogene Related To SRC, FGR, YES (FYN) ELISA KitFYN 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
FYN Oncogene Related To SRC, FGR, YES (FYN) ELISA KitFYN 适用: 人 pTyr530 Colorimetric Sandwich ELISA Cell Culture Lysate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FYN Oncogene Related To SRC/FGR/YES (FYN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FYN Oncogene Related To SRC/FGR/YES (FYN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FYN Oncogene Related To SRC/FGR/YES (FYN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FYN Oncogene Related To SRC/FGR/YES (FYN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FYN Oncogene Related To SRC/FGR/YES (FYN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FYN Oncogene Related To SRC/FGR/YES (FYN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all FYN ELISA试剂盒
- FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))
- 别名
- FYN Oncogene Related To SRC/FGR/YES (FYN 产品)
- 别名
- im:6908851 ELISA Kit, si:dkey-119m7.5 ELISA Kit, zgc:109996 ELISA Kit, c-fyn ELISA Kit, cfyn-a ELISA Kit, SLK ELISA Kit, SYN ELISA Kit, p59-FYN ELISA Kit, PKC ELISA Kit, fyn ELISA Kit, zgc:86720 ELISA Kit, AI448320 ELISA Kit, AW552119 ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase S homeolog ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase b ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase L homeolog ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase a ELISA Kit, Fyn proto-oncogene ELISA Kit, FYN ELISA Kit, fyn.S ELISA Kit, fynb ELISA Kit, fyn.L ELISA Kit, Fyn ELISA Kit, fyna ELISA Kit
- 背景
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Gene Name: FYN Oncogene Related To SRC/FGR/YES
Gene Aliases: SLK, SYN, c-Fyn, Src-like kinase
- 基因ID
- 2534
- UniProt
- P06241
- 途径
- JAK/STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling
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