FURIN ELISA 试剂盒
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- 抗原 See all FURIN ELISA试剂盒
- FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Furin (FUR). No significant cross-reactivity or interference between Furin (FUR) and analogues was observed.
- 灵敏度
- 0.053 ng/mL
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Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA Kit
FURIN 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 小鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 AA 108-715 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Furin (FUR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Furin (FUR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Furin (FUR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Furin (FUR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Furin (FUR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Furin (FUR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all FURIN ELISA试剂盒
- FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))
- 别名
- Furin (FURIN 产品)
- 别名
- FURIN ELISA Kit, furin ELISA Kit, si:dkey-281a24.5 ELISA Kit, FUR ELISA Kit, LOC100220259 ELISA Kit, PACE ELISA Kit, spc1 ELISA Kit, xfurin ELISA Kit, MGC82710 ELISA Kit, PCSK3 ELISA Kit, SPC1 ELISA Kit, 9130404I01Rik ELISA Kit, Fur ELISA Kit, Pcsk3 ELISA Kit, Pace ELISA Kit, furin, paired basic amino acid cleaving enzyme ELISA Kit, furin (paired basic amino acid cleaving enzyme) a ELISA Kit, furin (paired basic amino acid cleaving enzyme) b ELISA Kit, furin, paired basic amino acid cleaving enzyme L homeolog ELISA Kit, furin (paired basic amino acid cleaving enzyme) ELISA Kit, FURIN ELISA Kit, furina ELISA Kit, furinb ELISA Kit, furin ELISA Kit, furin.L ELISA Kit, Furin ELISA Kit
- 背景
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Gene Name: Furin
Gene Aliases: PACE, PCSK3, SPC1, Paired Basic Amino Acid Cleaving Enzyme, Dibasic-processing enzyme
- 途径
- Notch Signaling, Neurotrophin Signaling Pathway
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