FLT3 ELISA 试剂盒
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- 抗原 See all FLT3 ELISA试剂盒
- FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of FMS Like Tyrosine Kinase 3 (Flt3). No significant cross-reactivity or interference between FMS Like Tyrosine Kinase 3 (Flt3) and analogues was observed.
- 灵敏度
- 0.118 ng/mL
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Fms-Related tyrosine Kinase 3 (FLT3) ELISA Kit
FLT3 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Fms-Related tyrosine Kinase 3 (FLT3) ELISA KitFLT3 适用: 人 pTyr589 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
Fms-Related tyrosine Kinase 3 (FLT3) ELISA KitFLT3 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 (FLT3) ELISA KitFLT3 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 (FLT3) ELISA KitFLT3 适用: 兔 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FMS Like Tyrosine Kinase 3 (Flt3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FMS Like Tyrosine Kinase 3 (Flt3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FMS Like Tyrosine Kinase 3 (Flt3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FMS Like Tyrosine Kinase 3 (Flt3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 (Flt3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 (Flt3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all FLT3 ELISA试剂盒
- FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))
- 别名
- FMS Like Tyrosine Kinase 3 (FLT3 产品)
- 别名
- FLT3 ELISA Kit, B230315G04 ELISA Kit, CD135 ELISA Kit, Flk-2 ELISA Kit, Flk2 ELISA Kit, Flt-3 ELISA Kit, Ly72 ELISA Kit, wmfl ELISA Kit, FLK-2 ELISA Kit, FLK2 ELISA Kit, STK1 ELISA Kit, fms related tyrosine kinase 3 ELISA Kit, FMS-like tyrosine kinase 3 ELISA Kit, fms-related tyrosine kinase 3 ELISA Kit, FLT3 ELISA Kit, Flt3 ELISA Kit
- 背景
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Gene Name: FMS Like Tyrosine Kinase 3
Gene Aliases: CD135, STK1, FLK2, Fms-Related Tyrosine Kinase 3, Stem Cell Tyrosine Kinase 1, FL Cytokine Receptor, Fetal Liver Kinase-2
- 基因ID
- 2322
- UniProt
- P36888
- 途径
- RTK signaling
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