FBLN3 ELISA 试剂盒
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- 抗原 See all FBLN3 ELISA试剂盒
- FBLN3 (Fibulin 3 (FBLN3))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Urine
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Fibulin 3 (FBLN3). No significant cross-reactivity or interference between Fibulin 3 (FBLN3) and analogues was observed.
- 灵敏度
- 6.0 pg/mL
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Fibulin 3 (FBLN3) ELISA Kit
FBLN3 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Urine
Fibulin 3 (FBLN3) ELISA KitFBLN3 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibulin 3 (FBLN3) ELISA KitFBLN3 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
Fibulin 3 (FBLN3) ELISA KitFBLN3 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Fibulin 3 (FBLN3) ELISA KitFBLN3 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibulin 3 (FBLN3) ELISA KitFBLN3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Urine
Fibulin 3 (FBLN3) ELISA KitFBLN3 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Fibulin 3 (FBLN3) ELISA KitFBLN3 适用: 人 Extracellular Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibulin 3 (FBLN3) ELISA KitFBLN3 适用: 人 AA 18-493 Colorimetric Sandwich ELISA
Fibulin 3 (FBLN3) ELISA KitFBLN3 适用: 小鼠 Extracellular Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 3 (FBLN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibulin 3 (FBLN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 3 (FBLN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibulin 3 (FBLN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibulin 3 (FBLN3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibulin 3 (FBLN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all FBLN3 ELISA试剂盒
- FBLN3 (Fibulin 3 (FBLN3))
- 别名
- Fibulin 3 (FBLN3 产品)
- 别名
- DHRD ELISA Kit, DRAD ELISA Kit, FBLN3 ELISA Kit, FBNL ELISA Kit, FIBL-3 ELISA Kit, MLVT ELISA Kit, MTLV ELISA Kit, S1-5 ELISA Kit, T16 ELISA Kit, EGF containing fibulin like extracellular matrix protein 1 ELISA Kit, EGF-containing fibulin-like extracellular matrix protein 1 ELISA Kit, epidermal growth factor-containing fibulin-like extracellular matrix protein 1 ELISA Kit, EFEMP1 ELISA Kit, Efemp1 ELISA Kit
- 背景
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Gene Name: Fibulin 3
Gene Aliases: EFEMP1, DHRD, DRAD, FBNL, MLVT, MTLV, S1-5, EGF-Containing Fibulin-Like Extracellular Matrix Protein 1, Fibrillin-Like, Extracellular protein S1-5
- 途径
- EGFR Signaling Pathway
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