FGFR2 ELISA 试剂盒
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- 抗原 See all FGFR2 ELISA试剂盒
- FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor Receptor 2 (FGFR2). No significant cross-reactivity or interference between Fibroblast Growth Factor Receptor 2 (FGFR2) and analogues was observed.
- 灵敏度
- 12.2 pg/mL
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Fibroblast Growth Factor Receptor 2 (FGFR2) ELISA Kit
FGFR2 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor Receptor 2 (FGFR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor Receptor 2 (FGFR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor Receptor 2 (FGFR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor Receptor 2 (FGFR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor Receptor 2 (FGFR2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor Receptor 2 (FGFR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all FGFR2 ELISA试剂盒
- FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))
- 别名
- Fibroblast Growth Factor Receptor 2 (FGFR2 产品)
- 别名
- FGFR2 ELISA Kit, FGFR2IIIb ELISA Kit, bek ELISA Kit, bfr-1 ELISA Kit, cd332 ELISA Kit, cek3 ELISA Kit, cfd1 ELISA Kit, ect1 ELISA Kit, fgfr-2 ELISA Kit, jws ELISA Kit, k-sam ELISA Kit, kgfr ELISA Kit, tk14 ELISA Kit, tk25 ELISA Kit, xfgfr2 ELISA Kit, DKFZp469H1521 ELISA Kit, BBDS ELISA Kit, BEK ELISA Kit, BFR-1 ELISA Kit, CD332 ELISA Kit, CEK3 ELISA Kit, CFD1 ELISA Kit, ECT1 ELISA Kit, JWS ELISA Kit, K-SAM ELISA Kit, KGFR ELISA Kit, TK14 ELISA Kit, TK25 ELISA Kit, AU043015 ELISA Kit, AW556123 ELISA Kit, Bek ELISA Kit, Fgfr-2 ELISA Kit, Fgfr-7 ELISA Kit, Fgfr7 ELISA Kit, KGFRTr ELISA Kit, svs ELISA Kit, FGF-R ELISA Kit, fgfr72IIIb ELISA Kit, FGFR ELISA Kit, fc56c05 ELISA Kit, wu:fc56c05 ELISA Kit, fibroblast growth factor receptor 2 ELISA Kit, fibroblast growth factor receptor 2 L homeolog ELISA Kit, FGFR2 ELISA Kit, LOC100084641 ELISA Kit, fgfr2 ELISA Kit, Fgfr2 ELISA Kit, fgfr2.L ELISA Kit
- 背景
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Gene Name: Fibroblast Growth Factor Receptor 2
Gene Aliases: CD332, BEK, BFR1, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, Bacteria-Expressed Kinase,Keratinocyte Growth Factor Receptor, Craniofacial Dysostosis 1, Crouzon Syndrome
- 基因ID
- 2263
- UniProt
- P21802
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Growth Factor Binding
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