Fetal Hemoglobin ELISA 试剂盒
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- 抗原 See all Fetal Hemoglobin (HbF) products
- Fetal Hemoglobin (HbF)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 2.47 ng/mL - 200 ng/mL
- 最低检测浓度
- 2.47 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Fetal Hemoglobin (HBF). No significant cross-reactivity or interference between Fetal Hemoglobin (HBF) and analogues was observed.
- 灵敏度
- 0.99 ng/mL
- Featured
- Discover our best selling HbF ELISA Kit
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Fetal Hemoglobin (HbF) ELISA Kit
HbF 适用: 人 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Lysate, Plasma, Serum
Fetal Hemoglobin (HbF) ELISA KitHbF 适用: 人 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Cell Culture Supernatant, Plasma, Serum
Fetal Hemoglobin (HbF) ELISA KitHbF 适用: 人 Colorimetric Competition ELISA 125 ng/mL - 2000 ng/mL Plasma, Red Blood Cell Lysate, Serum
Fetal Hemoglobin (HbF) ELISA KitHbF 适用: 猴 Colorimetric Sandwich ELISA 12.5-800 ng/mL Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fetal Hemoglobin (HBF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fetal Hemoglobin (HBF) and unlabeled Fetal Hemoglobin (HBF) (Standards or samples) with the pre-coated antibody specific to Fetal Hemoglobin (HBF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fetal Hemoglobin (HBF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fetal Hemoglobin (HBF) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fetal Hemoglobin (HBF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fetal Hemoglobin (HBF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Fetal Hemoglobin (HbF) products
- Fetal Hemoglobin (HbF)
- 别名
- Fetal Hemoglobin (HbF 产品)
- 背景
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Gene Name: Fetal Hemoglobin
Gene Aliases: hemoglobin F, Foetal Haemoglobin
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