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Estrone CLIA Kit

适用: Various Species Chemiluminescent Competition ELISA 23.44 pg/mL - 6000 pg/mL Plasma, Serum
产品编号 ABIN5653996
发货至: 中国
  • 抗原 See all Estrone CLIA Kits
    Estrone
    适用
    • 5
    • 1
    Various Species
    检测方法
    Chemiluminescent
    实验类型
    Competition ELISA
    检测范围
    23.44 pg/mL - 6000 pg/mL
    最低检测浓度
    23.44 pg/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Estrone (E1). No significant cross-reactivity or interference between Estrone (E1) and analogues was observed.
    灵敏度
    7.96 pg/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    2 h
    板类型
    Pre-coated
    实验流程
    The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Estrone (E1). A competitive inhibition reaction is launched between biotin labeled Estrone (E1) and unlabeled Estrone (E1) (Standards or samples) with the pre-coated antibody specific to Estrone (E1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Estrone (E1) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Estrone (E1) level in the sample or standard.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrone (E1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrone (E1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all Estrone CLIA Kits
    Estrone
    Abstract
    Estrone 产品
    物质类
    Amino Acid
    背景

    Gene Name: Estrone

    Gene Aliases: Oestrone

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