Estrone CLIA Kit
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- 抗原 See all Estrone CLIA Kits
- Estrone
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适用
- Various Species
- 检测方法
- Chemiluminescent
- 实验类型
- Competition ELISA
- 检测范围
- 23.44 pg/mL - 6000 pg/mL
- 最低检测浓度
- 23.44 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Estrone (E1). No significant cross-reactivity or interference between Estrone (E1) and analogues was observed.
- 灵敏度
- 7.96 pg/mL
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Estrone ELISA Kit
适用: Various Species Colorimetric Competition ELISA 125 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estrone ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 0.156 pmol/mL - 10 pmol/mL Plasma, Serum, Tissue Homogenate
Estrone ELISA KitSmall Sample 适用: Various Species Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estrone ELISA Kit适用: Various Species Colorimetric Sandwich ELISA Fecal, Tissue Culture Medium, Urine
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Estrone (E1). A competitive inhibition reaction is launched between biotin labeled Estrone (E1) and unlabeled Estrone (E1) (Standards or samples) with the pre-coated antibody specific to Estrone (E1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Estrone (E1) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Estrone (E1) level in the sample or standard.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrone (E1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrone (E1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Estrone CLIA Kits
- Estrone
- Abstract
- Estrone 产品
- 物质类
- Amino Acid
- 背景
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Gene Name: Estrone
Gene Aliases: Oestrone
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