EBI3 CLIA Kit
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- 抗原 See all EBI3 (IL-27b) CLIA Kits
- EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))
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适用
- 小鼠
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 156.25 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Epstein Barr Virus Induced Protein 3 (EBI3). No significant cross-reactivity or interference between Epstein Barr Virus Induced Protein 3 (EBI3) and analogues was observed.
- 灵敏度
- 5.79 pg/mL
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Interleukin-27 subunit beta (IL-27b) ELISA Kit
IL-27b 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin-27 subunit beta (IL-27b) ELISA KitIL-27b 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Epstein Barr Virus Induced Protein 3 (EBI3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epstein Barr Virus Induced Protein 3 (EBI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epstein Barr Virus Induced Protein 3 (EBI3) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epstein Barr Virus Induced Protein 3 (EBI3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epstein Barr Virus Induced Protein 3 (EBI3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all EBI3 (IL-27b) CLIA Kits
- EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))
- 别名
- Epstein Barr Virus Induced Protein 3 (IL-27b 产品)
- 别名
- IL-27B CLIA Kit, IL27B CLIA Kit, EBI3 CLIA Kit, il-27rb CLIA Kit, si:dkey-97a13.4 CLIA Kit, EBI-3 CLIA Kit, IL-27 CLIA Kit, Epstein-Barr virus induced 3 CLIA Kit, Epstein-Barr virus induced gene 3 CLIA Kit, EBI3 CLIA Kit, ebi3 CLIA Kit, Ebi3 CLIA Kit
- 物质类
- Viral Protein
- 背景
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Gene Name: Epstein Barr Virus Induced Protein 3
Gene Aliases: IL27B, IL27-B, Interleukin 27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein
- 基因ID
- 50498
- UniProt
- O35228
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