Elastase 3B ELISA 试剂盒
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- 抗原 See all Elastase 3B (ELA3B) ELISA试剂盒
- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Elastase 3B (ELA3B). No significant cross-reactivity or interference between Elastase 3B (ELA3B) and analogues was observed.
- 灵敏度
- 0.135 ng/mL
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Elastase 3B, Pancreatic (ELA3B) ELISA Kit
ELA3B 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Elastase 3B, Pancreatic (ELA3B) ELISA KitELA3B 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Elastase 3B, Pancreatic (ELA3B) ELISA KitELA3B 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3B (ELA3B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 3B (ELA3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 3B (ELA3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 3B (ELA3B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastase 3B (ELA3B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastase 3B (ELA3B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Elastase 3B (ELA3B) ELISA试剂盒
- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
- 别名
- Elastase 3B (ELA3B 产品)
- 别名
- CBPP ELISA Kit, E1 ELISA Kit, EL-1 ELISA Kit, ELA3B ELISA Kit, 0910001F22Rik ELISA Kit, 2310074F01Rik ELISA Kit, AI504000 ELISA Kit, Ela3 ELISA Kit, Ela3b ELISA Kit, ela3b ELISA Kit, MGC82867 ELISA Kit, ELA1 ELISA Kit, Cela3b ELISA Kit, CELA3B ELISA Kit, chymotrypsin like elastase family member 3B ELISA Kit, chymotrypsin-like elastase family, member 3B ELISA Kit, elastase 3B, pancreatic ELISA Kit, proproteinase E ELISA Kit, chymotrypsin like elastase family member 3B L homeolog ELISA Kit, chymotrypsin-like elastase family member 3B ELISA Kit, CELA3B ELISA Kit, Cela3b ELISA Kit, ela3b ELISA Kit, LOC478196 ELISA Kit, cela3b.L ELISA Kit, LOC100717936 ELISA Kit, LOC100071692 ELISA Kit
- 背景
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Gene Name: Elastase 3B
Gene Aliases: CELA3B, Chymotrypsin Like Elastase Family Member 3B, Protease E, Fecal Elastase, Elastase 1, Cholesterol-Binding Pancreatic Protease, Pancreatic Endopeptidase E
- 基因ID
- 23436
- UniProt
- P08861
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