p300 ELISA 试剂盒
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- 抗原 See all p300 (EP300) ELISA试剂盒
- p300 (EP300) (E1A Binding Protein P300 (EP300))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of E1A Binding Protein P300 (EP300). No significant cross-reactivity or interference between E1A Binding Protein P300 (EP300) and analogues was observed.
- 灵敏度
- 0.055 ng/mL
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E1A Binding Protein P300 (EP300) ELISA Kit
EP300 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
E1A Binding Protein P300 (EP300) ELISA KitEP300 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
E1A Binding Protein P300 (EP300) ELISA KitEP300 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E1A Binding Protein P300 (EP300). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to E1A Binding Protein P300 (EP300). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E1A Binding Protein P300 (EP300), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E1A Binding Protein P300 (EP300) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E1A Binding Protein P300 (EP300) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E1A Binding Protein P300 (EP300) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all p300 (EP300) ELISA试剂盒
- p300 (EP300) (E1A Binding Protein P300 (EP300))
- 别名
- E1A Binding Protein P300 (EP300 产品)
- 别名
- KAT3B ELISA Kit, RSTS2 ELISA Kit, p300 ELISA Kit, A430090G16 ELISA Kit, A730011L11 ELISA Kit, p300 HAT ELISA Kit, E1A binding protein p300 ELISA Kit, histone acetyltransferase p300 ELISA Kit, EP300 ELISA Kit, LOC446056 ELISA Kit, ep300 ELISA Kit, Ep300 ELISA Kit
- 背景
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Gene Name: E1A Binding Protein P300
Gene Aliases: KAT3B, p300, Histone Acetyltransferase p300
- 基因ID
- 328572
- UniProt
- B2RWS6
- 途径
- p53 Pathway, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
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