DOT1L ELISA 试剂盒
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- 抗原 See all DOT1L ELISA试剂盒
- DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of DOT1 Like, Histone H3 Methyltransferase (DOT1L). No significant cross-reactivity or interference between DOT1 Like, Histone H3 Methyltransferase (DOT1L) and analogues was observed.
- 灵敏度
- 0.058 ng/mL
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DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) ELISA Kit
DOT1L 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) ELISA KitDOT1L 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DOT1 Like, Histone H3 Methyltransferase (DOT1L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DOT1 Like, Histone H3 Methyltransferase (DOT1L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DOT1 Like, Histone H3 Methyltransferase (DOT1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DOT1 Like, Histone H3 Methyltransferase (DOT1L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DOT1 Like, Histone H3 Methyltransferase (DOT1L) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DOT1 Like, Histone H3 Methyltransferase (DOT1L) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all DOT1L ELISA试剂盒
- DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))
- 别名
- DOT1 Like, Histone H3 Methyltransferase (DOT1L 产品)
- 别名
- NV11307 ELISA Kit, DOT1L ELISA Kit, DOT1 ELISA Kit, KMT4 ELISA Kit, A630076O07 ELISA Kit, AW907654 ELISA Kit, Dot1 ELISA Kit, mDot1 ELISA Kit, histone-lysine N-methyltransferase, H3 lysine-79 specific ELISA Kit, DOT1 like histone lysine methyltransferase ELISA Kit, histone methyltransferase ELISA Kit, DOT1-like, histone H3 methyltransferase (S. cerevisiae) ELISA Kit, LOC100116648 ELISA Kit, DOT1L ELISA Kit, DOT1 ELISA Kit, Dot1l ELISA Kit
- 背景
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Gene Name: DOT1 Like, Histone H3 Methyltransferase
Gene Aliases: DOT1, KMT4, Histone-lysine N-methyltransferase, H3 lysine-79 specific, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4
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