DEFB103A ELISA 试剂盒
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- 抗原 See all DEFB103A ELISA试剂盒
- DEFB103A (Defensin, beta 103A (DEFB103A))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 46.88 pg/mL - 3000 pg/mL
- 最低检测浓度
- 46.88 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Defensin Beta 103A (DEFb103A). No significant cross-reactivity or interference between Defensin Beta 103A (DEFb103A) and analogues was observed.
- 灵敏度
- 19.54 pg/mL
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Defensin, beta 103A (DEFB103A) ELISA Kit
DEFB103A 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Defensin, beta 103A (DEFB103A) ELISA KitDEFB103A 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Defensin, beta 103A (DEFB103A) ELISA KitDEFB103A 适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103A (DEFb103A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 103A (DEFb103A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 103A (DEFb103A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 103A (DEFb103A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 103A (DEFb103A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 103A (DEFb103A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all DEFB103A ELISA试剂盒
- DEFB103A (Defensin, beta 103A (DEFB103A))
- 别名
- Defensin Beta 103A (DEFB103A 产品)
- 别名
- BD-3 ELISA Kit, DEFB-3 ELISA Kit, DEFB103 ELISA Kit, DEFB3 ELISA Kit, HBD3 ELISA Kit, HBP-3 ELISA Kit, HBP3 ELISA Kit, hBD-3 ELISA Kit, DEFB103B ELISA Kit, defensin beta 103A ELISA Kit, defensin beta 103B ELISA Kit, defensin, beta 103A ELISA Kit, beta-defensin 103 ELISA Kit, DEFB103A ELISA Kit, DEFB103B ELISA Kit, LOC694235 ELISA Kit, LOC100359096 ELISA Kit
- 背景
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Gene Name: Defensin Beta 103A
Gene Aliases: DEFB103, DEFB3, HBD-3, HBD3, HBP-3, HBP3, Beta-defensin 3, Defensin-like protein
- 基因ID
- 414325
- UniProt
- P81534
- 途径
- Production of Molecular Mediator of Immune Response
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