CYBB ELISA 试剂盒
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- 抗原 See all CYBB ELISA试剂盒
- CYBB (Cytochrome B-245, beta Polypeptide (CYBB))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Cytochrome b-245 Beta Polypeptide (CYBb). No significant cross-reactivity or interference between Cytochrome b-245 Beta Polypeptide (CYBb) and analogues was observed.
- 灵敏度
- 0.055 ng/mL
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Cytochrome B-245, beta Polypeptide (CYBB) ELISA Kit
CYBB 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cytochrome B-245, beta Polypeptide (CYBB) ELISA KitCYBB 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cytochrome B-245, beta Polypeptide (CYBB) ELISA KitCYBB 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome b-245 Beta Polypeptide (CYBb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome b-245 Beta Polypeptide (CYBb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome b-245 Beta Polypeptide (CYBb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome b-245 Beta Polypeptide (CYBb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome b-245 Beta Polypeptide (CYBb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome b-245 Beta Polypeptide (CYBb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CYBB ELISA试剂盒
- CYBB (Cytochrome B-245, beta Polypeptide (CYBB))
- 别名
- Cytochrome b-245 Beta Polypeptide (CYBB 产品)
- 别名
- zgc:64144 ELISA Kit, cgd ELISA Kit, gp91-1 ELISA Kit, gp91-phox ELISA Kit, gp91phox ELISA Kit, nox2 ELISA Kit, NOX2 ELISA Kit, cybb ELISA Kit, NOX1 ELISA Kit, AMCBX2 ELISA Kit, CGD ELISA Kit, GP91-1 ELISA Kit, GP91-PHOX ELISA Kit, GP91PHOX ELISA Kit, p91-PHOX ELISA Kit, Nox2 ELISA Kit, C88302 ELISA Kit, CGD91-phox ELISA Kit, Cgd ELISA Kit, Cyd ELISA Kit, Gp91-phox ELISA Kit, CYBB ELISA Kit, cytochrome b-245, beta polypeptide (chronic granulomatous disease) ELISA Kit, cytochrome b-245, beta polypeptide ELISA Kit, cytochrome b-245 heavy chain ELISA Kit, cytochrome b-245, beta polypeptide L homeolog ELISA Kit, cytochrome b-245 beta polypeptide ELISA Kit, cytochrome b-245 beta chain ELISA Kit, NADPH oxidase heavy chain subunit ELISA Kit, cybb ELISA Kit, CYBB ELISA Kit, cybb.L ELISA Kit, Cybb ELISA Kit, GP91-PHOX ELISA Kit
- 背景
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Gene Name: Cytochrome b-245 Beta Polypeptide
Gene Aliases: CGD, GP91PHOX, NOX2, Neutrophil cytochrome b91, Cytochrome b558 beta, Heme-binding membrane glycoprotein gp91phox, Superoxide-generating NADPH oxidase heavy chain
- 基因ID
- 1536
- UniProt
- P04839
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