CYR61 ELISA 试剂盒
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- 抗原 See all CYR61 ELISA试剂盒
- CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 6.25 pg/mL - 400 pg/mL
- 最低检测浓度
- 6.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). No significant cross-reactivity or interference between Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) and analogues was observed.
- 灵敏度
- 2.36 pg/mL
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Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA Kit
CYR61 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Saliva, Serum, Tissue Homogenate
Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA KitCYR61 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA KitCYR61 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA KitCYR61 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA KitCYR61 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA KitCYR61 适用: 人 AA 22-381 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA KitCYR61 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CYR61 ELISA试剂盒
- CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))
- 别名
- Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61 产品)
- 别名
- CCN1 ELISA Kit, GIG1 ELISA Kit, IGFBP10 ELISA Kit, ccn1 ELISA Kit, gig1 ELISA Kit, Xcyr61 ELISA Kit, igfbp10 ELISA Kit, MGC69281 ELISA Kit, MGC69539 ELISA Kit, cyr61-l2 ELISA Kit, zgc:158158 ELISA Kit, CYR61 ELISA Kit, AI325051 ELISA Kit, Igfbp10 ELISA Kit, cysteine rich angiogenic inducer 61 ELISA Kit, cysteine-rich, angiogenic inducer, 61 ELISA Kit, cysteine-rich, angiogenic inducer, 61 like 2 ELISA Kit, cysteine rich protein 61 ELISA Kit, cysteine rich angiogenic inducer 61 S homeolog ELISA Kit, CYR61 ELISA Kit, Cyr61 ELISA Kit, cyr61 ELISA Kit, cyr61l2 ELISA Kit, cyr61.S ELISA Kit
- 背景
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Gene Name: Cysteine Rich Protein, Angiogenic Inducer 61
Gene Aliases: CCN1, GIG1, IGFBP10, CCN family member 1, Insulin-like growth factor-binding protein 10
- 基因ID
- 16007
- UniProt
- P18406
- 途径
- Positive Regulation of Endopeptidase Activity, Growth Factor Binding
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