Cyclic GMP ELISA 试剂盒
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- 抗原 See all Cyclic GMP (cGMP) ELISA试剂盒
- Cyclic GMP (cGMP)
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适用
- Various Species
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 123.5 pg/mL - 10000 pg/mL
- 最低检测浓度
- 123.5 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Cyclic Guanosine Monophosphate (cGMP). No significant cross-reactivity or interference between Cyclic Guanosine Monophosphate (cGMP) and analogues was observed.
- 灵敏度
- 45.2 pg/mL
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Cyclic GMP (cGMP) ELISA Kit
cGMP 适用: Various Species Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cyclic Guanosine Monophosphate (cGMP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cyclic Guanosine Monophosphate (cGMP) and unlabeled Cyclic Guanosine Monophosphate (cGMP) (Standards or samples) with the pre-coated antibody specific to Cyclic Guanosine Monophosphate (cGMP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cyclic Guanosine Monophosphate (cGMP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cyclic Guanosine Monophosphate (cGMP) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclic Guanosine Monophosphate (cGMP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclic Guanosine Monophosphate (cGMP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Cyclic GMP (cGMP) ELISA试剂盒
- Cyclic GMP (cGMP)
- 别名
- Cyclic Guanosine Monophosphate (cGMP 产品)
- 物质类
- Chemical
- 背景
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Gene Name: Cyclic Guanosine Monophosphate
Gene Aliases: c-GMP, 3',5'-Cyclic GMP, Cyclic 3',5'-GMP, Guanosine 3',5'-Cyclic Phosphate
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