Adipsin ELISA 试剂盒
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- 抗原 See all Adipsin (CFD) ELISA试剂盒
- Adipsin (CFD) (Complement Factor D (CFD))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Complement Factor D (CFD). No significant cross-reactivity or interference between Complement Factor D (CFD) and analogues was observed.
- 灵敏度
- 0.069 ng/mL
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Complement Factor D (CFD) ELISA Kit
CFD 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Factor D (CFD) ELISA KitCFD 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Complement Factor D (CFD) ELISA KitCFD 适用: 小鼠 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Factor D (CFD) ELISA KitCFD 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Complement Factor D (CFD) ELISA KitCFD 适用: 人 AA 26-253 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Complement Factor D (CFD) ELISA KitCFD 适用: 小鼠 AA 26-259 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Complement Factor D (CFD) ELISA KitCFD 适用: 人 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
Complement Factor D (CFD) ELISA KitCFD 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Milk, Plasma, Saliva, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor D (CFD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor D (CFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor D (CFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor D (CFD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor D (CFD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor D (CFD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Adipsin (CFD) ELISA试剂盒
- Adipsin (CFD) (Complement Factor D (CFD))
- 别名
- Complement Factor D (CFD 产品)
- 别名
- ADIPSIN ELISA Kit, ADN ELISA Kit, DF ELISA Kit, PFD ELISA Kit, Adn ELISA Kit, Df ELISA Kit, EVE ELISA Kit, CFD ELISA Kit, adipsin ELISA Kit, pfd ELISA Kit, cfdl ELISA Kit, wu:fb61f12 ELISA Kit, zgc:109940 ELISA Kit, 10.5 ELISA Kit, 10.9 ELISA Kit, 14.10 ELISA Kit, 20.35 ELISA Kit, CG2328 ELISA Kit, Dmel\\CG2328 ELISA Kit, E(eve) ELISA Kit, Eve ELISA Kit, F ELISA Kit, V ELISA Kit, VI ELISA Kit, eve2 ELISA Kit, even ELISA Kit, l(2)46CFg ELISA Kit, l(2)46CFh ELISA Kit, l(2)46CFj ELISA Kit, l(2)46CFp ELISA Kit, l(2)46Ce ELISA Kit, l(2)46Cg ELISA Kit, complement factor D ELISA Kit, complement factor D (adipsin) ELISA Kit, complement factor D (adipsin) L homeolog ELISA Kit, even skipped ELISA Kit, CFD ELISA Kit, Cfd ELISA Kit, cfd.L ELISA Kit, cfd ELISA Kit, eve ELISA Kit
- 背景
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Gene Name: Complement Factor D
Gene Aliases: CF-D, DF, PFD, ADN, Adipsin, Properdin Factor D, C3 convertase activator
- 基因ID
- 1675
- UniProt
- P00746
- 途径
- Complement System
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