CD131 ELISA 试剂盒
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- 抗原 See all CD131 (CSF2RB) ELISA试剂盒
- CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Colony Stimulating Factor 2 Receptor Beta (CSF2Rb). No significant cross-reactivity or interference between Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) and analogues was observed.
- 灵敏度
- 0.113 ng/mL
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Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) ELISA Kit
CSF2RB 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) ELISA KitCSF2RB 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2 Receptor Beta (CSF2Rb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 2 Receptor Beta (CSF2Rb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 2 Receptor Beta (CSF2Rb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CD131 (CSF2RB) ELISA试剂盒
- CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))
- 别名
- Colony Stimulating Factor 2 Receptor Beta (CSF2RB 产品)
- 别名
- Csf2rb1 ELISA Kit, CSF2RB ELISA Kit, cd131 ELISA Kit, cdw131 ELISA Kit, il3rb ELISA Kit, il5rb ELISA Kit, il-3rbc ELISA Kit, CD131 ELISA Kit, CDw131 ELISA Kit, IL3RB ELISA Kit, IL5RB ELISA Kit, SMDP5 ELISA Kit, AI848964 ELISA Kit, AIC2B ELISA Kit, Bc ELISA Kit, Csfgmrb ELISA Kit, Il3r ELISA Kit, Il3rb ELISA Kit, Il3rb1 ELISA Kit, Il5rb ELISA Kit, colony stimulating factor 2 receptor beta common subunit ELISA Kit, colony stimulating factor 2 receptor beta common subunit S homeolog ELISA Kit, cytokine receptor common subunit beta-like ELISA Kit, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) ELISA Kit, CSF2RB ELISA Kit, Csf2rb ELISA Kit, csf2rb.S ELISA Kit, CRCBL ELISA Kit, csf2rb ELISA Kit
- 背景
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Gene Name: Colony Stimulating Factor 2 Receptor Beta
Gene Aliases: CD131, CSF2R-B, IL3-RB, IL3RB, CSF2-RB, Colony Stimulating Factor 2 Receptor,Beta,Low-Affinity(granulocyte-Macrophage), GM-CSF/IL-3/IL-5 receptor common beta subunit
- 基因ID
- 1439
- UniProt
- P32927
- 途径
- JAK/STAT Signaling
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