COL1A1 ELISA 试剂盒
-
- 抗原 See all COL1A1 ELISA试剂盒
- COL1A1 (Collagen, Type I, alpha 1 (COL1A1))
-
适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 1 (COL1a1). No significant cross-reactivity or interference between Collagen Type I Alpha 1 (COL1a1) and analogues was observed.
- 灵敏度
- 13.7 pg/mL
- Featured
- Discover our best selling COL1A1 ELISA Kit
- Top Product
- Discover our top product COL1A1 ELISA Kit
-
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 1 (COL1a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 1 (COL1a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 1 (COL1a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I Alpha 1 (COL1a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all COL1A1 ELISA试剂盒
- COL1A1 (Collagen, Type I, alpha 1 (COL1A1))
- 别名
- Collagen Type I Alpha 1 (COL1A1 产品)
- 别名
- OI4 ELISA Kit, Col1a-1 ELISA Kit, Cola-1 ELISA Kit, Cola1 ELISA Kit, Mov-13 ELISA Kit, Mov13 ELISA Kit, COLIA1 ELISA Kit, col1a1 ELISA Kit, MGC52532 ELISA Kit, COL1A1 ELISA Kit, COL1A2 ELISA Kit, alpha1(I) ELISA Kit, cb21 ELISA Kit, sb:cb21 ELISA Kit, sb:cb384 ELISA Kit, wu:fa99c12 ELISA Kit, wu:fb02c06 ELISA Kit, alpha3(I) ELISA Kit, col1a3 ELISA Kit, hm:zeh0348 ELISA Kit, wu:fa95h05 ELISA Kit, wu:fd02a10 ELISA Kit, wu:fj59a10 ELISA Kit, collagen type I alpha 1 chain ELISA Kit, collagen, type I, alpha 1 ELISA Kit, collagen, type I, alpha 1 S homeolog ELISA Kit, collagen alpha-1(I) chain ELISA Kit, collagen type I alpha 2 chain ELISA Kit, collagen, type I, alpha 1a ELISA Kit, collagen 1a1 ELISA Kit, collagen, type I, alpha 1b ELISA Kit, COL1A1 ELISA Kit, Col1a1 ELISA Kit, col1a1.S ELISA Kit, LOC397571 ELISA Kit, COL1A2 ELISA Kit, col1a1a ELISA Kit, col1a1 ELISA Kit, col1a1b ELISA Kit
- 背景
-
Gene Name: Collagen Type I Alpha 1
Gene Aliases: COL-1A1, COL1-A1, COL1A-1, OI4, Collagen Alpha-1(I)chain
- 途径
- Sensory Perception of Sound, Autophagy, Growth Factor Binding
-