COQ10B ELISA 试剂盒
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- 抗原 See all COQ10B ELISA试剂盒
- COQ10B (Coenzyme Q10 Homolog B (COQ10B))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Coenzyme Q10 Homolog B (COQ10B). No significant cross-reactivity or interference between Coenzyme Q10 Homolog B (COQ10B) and analogues was observed.
- 灵敏度
- 0.134 ng/mL
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Coenzyme Q10 Homolog B (COQ10B) ELISA Kit
COQ10B 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Coenzyme Q10 Homolog B (COQ10B) ELISA KitCOQ10B 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Coenzyme Q10 Homolog B (COQ10B) ELISA KitCOQ10B 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coenzyme Q10 Homolog B (COQ10B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coenzyme Q10 Homolog B (COQ10B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coenzyme Q10 Homolog B (COQ10B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coenzyme Q10 Homolog B (COQ10B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coenzyme Q10 Homolog B (COQ10B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coenzyme Q10 Homolog B (COQ10B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all COQ10B ELISA试剂盒
- COQ10B (Coenzyme Q10 Homolog B (COQ10B))
- 别名
- Coenzyme Q10 Homolog B (COQ10B 产品)
- 别名
- 1500041J02Rik ELISA Kit, 1700030I21Rik ELISA Kit, 9530077A17Rik ELISA Kit, AV002237 ELISA Kit, AW742641 ELISA Kit, RGD1359509 ELISA Kit, fc38f07 ELISA Kit, im:6912267 ELISA Kit, wu:fc38f07 ELISA Kit, zgc:112103 ELISA Kit, coenzyme Q10B ELISA Kit, COQ10B ELISA Kit, Coq10b ELISA Kit, coq10b ELISA Kit
- 背景
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Gene Name: Coenzyme Q10 Homolog B
Gene Aliases: Coenzyme Q-binding protein COQ10 homolog B, mitochondrial
- 基因ID
- 80219
- UniProt
- Q9H8M1
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