CERK ELISA 试剂盒
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- CERK (Ceramide Kinase (CERK))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 3.12 ng/mL - 200 ng/mL
- 最低检测浓度
- 3.12 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Ceramide Kinase (CERK). No significant cross-reactivity or interference between Ceramide Kinase (CERK) and analogues was observed.
- 灵敏度
- 1.14 ng/mL
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Ceramide Kinase (CERK) ELISA Kit
CERK 适用: 人 Colorimetric Competition ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Ceramide Kinase (CERK) ELISA KitCERK 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ceramide Kinase (CERK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ceramide Kinase (CERK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ceramide Kinase (CERK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ceramide Kinase (CERK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ceramide Kinase (CERK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ceramide Kinase (CERK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CERK ELISA试剂盒
- CERK (Ceramide Kinase (CERK))
- 别名
- Ceramide Kinase (CERK 产品)
- 别名
- AI848610 ELISA Kit, D330016D08Rik ELISA Kit, mKIAA1646 ELISA Kit, LK4 ELISA Kit, dA59H18.2 ELISA Kit, dA59H18.3 ELISA Kit, hCERK ELISA Kit, CERK ELISA Kit, si:ch211-162i19.1 ELISA Kit, MGC81777 ELISA Kit, CG16708 ELISA Kit, DCERK ELISA Kit, Dcerk ELISA Kit, Dmel\\CG16708 ELISA Kit, dcerk ELISA Kit, ceramide kinase ELISA Kit, ceramide kinase S homeolog ELISA Kit, Ceramide kinase ELISA Kit, Cerk ELISA Kit, CERK ELISA Kit, cerk ELISA Kit, cerk.S ELISA Kit, CpipJ_CPIJ003342 ELISA Kit, LOAG_11245 ELISA Kit, LOC5579301 ELISA Kit, Bm1_50005 ELISA Kit
- 背景
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Gene Name: Ceramide Kinase
Gene Aliases: LK4, hCERK, Lipid kinase 4, Acylsphingosine kinase
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