CTNNA1 ELISA 试剂盒
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- 抗原 See all CTNNA1 ELISA试剂盒
- CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Catenin Alpha 1 (CTNNa1). No significant cross-reactivity or interference between Catenin Alpha 1 (CTNNa1) and analogues was observed.
- 灵敏度
- 0.054 ng/mL
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Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1) ELISA Kit
CTNNA1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catenin Alpha 1 (CTNNa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catenin Alpha 1 (CTNNa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catenin Alpha 1 (CTNNa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catenin Alpha 1 (CTNNa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CTNNA1 ELISA试剂盒
- CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))
- 别名
- Catenin Alpha 1 (CTNNA1 产品)
- 别名
- CAP102 ELISA Kit, 2010010M04Rik ELISA Kit, AA517462 ELISA Kit, AI988031 ELISA Kit, Catna1 ELISA Kit, CG17947 ELISA Kit, CT39986 ELISA Kit, Dalpha-Cat ELISA Kit, Dalpha-cat ELISA Kit, DalphaC ELISA Kit, Dalphacat ELISA Kit, Dmcatp ELISA Kit, Dmel\\CG17947 ELISA Kit, a-Cat ELISA Kit, a-cat ELISA Kit, alpha-CAT ELISA Kit, alpha-cat ELISA Kit, alpha-catenin ELISA Kit, alpha-ctn ELISA Kit, alphaCAT ELISA Kit, alphaCat ELISA Kit, alphacat ELISA Kit, Alpha-Cat ELISA Kit, dgri_GLEANR_3829 ELISA Kit, DgriGH19548 ELISA Kit, GH19548 ELISA Kit, ctnna ELISA Kit, cap102 ELISA Kit, catna1 ELISA Kit, ctnna1 ELISA Kit, catenin alpha 1 ELISA Kit, catenin (cadherin associated protein), alpha 1 ELISA Kit, alpha Catenin ELISA Kit, catenin alpha-1 ELISA Kit, catenin (cadherin-associated protein), alpha 1 ELISA Kit, catenin alpha 1 S homeolog ELISA Kit, CTNNA1 ELISA Kit, Ctnna1 ELISA Kit, alpha-Cat ELISA Kit, Dgri\alpha-Cat ELISA Kit, Tsp_10127 ELISA Kit, ctnna1 ELISA Kit, ctnna1.S ELISA Kit
- 背景
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Gene Name: Catenin Alpha 1
Gene Aliases: CTNN-A1, aCat, CAP102, Cadherin Associated Protein Alpha 1, Alpha E-catenin, Renal carcinoma antigen NY-REN-13
- 基因ID
- 1495
- UniProt
- P35221
- 途径
- Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location
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