Casein Kinase 1 delta ELISA 试剂盒
-
- 抗原 See all Casein Kinase 1 delta (CSNK1D) ELISA试剂盒
- Casein Kinase 1 delta (CSNK1D) (Casein Kinase 1, delta (CSNK1D))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Casein Kinase 1 Delta (CSNK1d). No significant cross-reactivity or interference between Casein Kinase 1 Delta (CSNK1d) and analogues was observed.
- 灵敏度
- 0.052 ng/mL
- Featured
- Discover our best selling CSNK1D ELISA Kit
- Top Product
- Discover our top product CSNK1D ELISA Kit
-
Casein Kinase 1, delta (CSNK1D) ELISA Kit
CSNK1D 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Casein Kinase 1, delta (CSNK1D) ELISA KitCSNK1D 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Kinase 1 Delta (CSNK1d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Casein Kinase 1 Delta (CSNK1d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Kinase 1 Delta (CSNK1d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Casein Kinase 1 Delta (CSNK1d) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Casein Kinase 1 Delta (CSNK1d) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Casein Kinase 1 Delta (CSNK1d) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all Casein Kinase 1 delta (CSNK1D) ELISA试剂盒
- Casein Kinase 1 delta (CSNK1D) (Casein Kinase 1, delta (CSNK1D))
- 别名
- Casein Kinase 1 Delta (CSNK1D 产品)
- 别名
- ASPS ELISA Kit, CKIdelta ELISA Kit, FASPS2 ELISA Kit, HCKID ELISA Kit, 1200006A05Rik ELISA Kit, AA409348 ELISA Kit, D930010H05Rik ELISA Kit, CKI-delta ELISA Kit, CKId ELISA Kit, csnk1d ELISA Kit, wu:fb69b03 ELISA Kit, wu:fj97a05 ELISA Kit, zgc:77627 ELISA Kit, CSNK1D ELISA Kit, ckIdelta ELISA Kit, Csnk1d ELISA Kit, csnk1dl ELISA Kit, wu:fb50c04 ELISA Kit, zgc:63971 ELISA Kit, casein kinase 1 delta ELISA Kit, casein kinase 1, delta ELISA Kit, casein kinase 1, delta b ELISA Kit, casein kinase 1 delta S homeolog ELISA Kit, casein kinase 1, delta a ELISA Kit, CSNK1D ELISA Kit, Csnk1d ELISA Kit, csnk1d ELISA Kit, csnk1db ELISA Kit, Bm1_37175 ELISA Kit, csnk1d.S ELISA Kit, csnk1da ELISA Kit
- 背景
-
Gene Name: Casein Kinase 1 Delta
Gene Aliases: HCKID, Tau-protein kinase CSNK1D, Casein kinase I isoform delta
- 基因ID
- 1453
- UniProt
- P48730
- 途径
- Hedgehog Signaling, M Phase
-
