CES1 ELISA 试剂盒
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- 抗原 See all CES1 ELISA试剂盒
- CES1 (Carboxylesterase 1 (CES1))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Carboxylesterase 1 (CES1). No significant cross-reactivity or interference between Carboxylesterase 1 (CES1) and analogues was observed.
- 灵敏度
- 14.1 pg/mL
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Carboxylesterase 1 (CES1) ELISA Kit
CES1 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Carboxylesterase 1 (CES1) ELISA KitCES1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxylesterase 1 (CES1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxylesterase 1 (CES1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxylesterase 1 (CES1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxylesterase 1 (CES1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxylesterase 1 (CES1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxylesterase 1 (CES1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CES1 ELISA试剂盒
- CES1 (Carboxylesterase 1 (CES1))
- 别名
- Carboxylesterase 1 (CES1 产品)
- 别名
- ACAT ELISA Kit, CE-1 ELISA Kit, CEH ELISA Kit, CES2 ELISA Kit, HMSE ELISA Kit, HMSE1 ELISA Kit, PCE-1 ELISA Kit, REH ELISA Kit, SES1 ELISA Kit, TGH ELISA Kit, hCE-1 ELISA Kit, CESDD1 ELISA Kit, CES1 ELISA Kit, CES-K1 ELISA Kit, APLE ELISA Kit, PMPMEase ELISA Kit, CES ELISA Kit, carboxylesterase 1 ELISA Kit, liver carboxylesterase 1 ELISA Kit, liver carboxylesterase ELISA Kit, carboxylesterase 1 (monocyte/macrophage serine esterase 1) ELISA Kit, CES1 ELISA Kit, CpipJ_CPIJ013026 ELISA Kit, CpipJ_CPIJ016339 ELISA Kit, LOC100009551 ELISA Kit, LOC454097 ELISA Kit, LOC699486 ELISA Kit, LOC100050915 ELISA Kit
- 背景
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Gene Name: Carboxylesterase 1
Gene Aliases: ACAT, CEH, CES2, HMSE1, PCE1, SES1, TGH, Retinyl ester hydrolase, Serine esterase 1, Triacylglycerol hydrolase, Egasyn, Cocaine carboxylesterase, Liver Carboxylesterase 1
- UniProt
- Q63108
- 途径
- Monocarboxylic Acid Catabolic Process
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