CAMK2B ELISA 试剂盒
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- 抗原 See all CAMK2B ELISA试剂盒
- CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) and analogues was observed.
- 灵敏度
- 0.113 ng/mL
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Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) ELISA Kit
CAMK2B 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) ELISA KitCAMK2B 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CAMK2B ELISA试剂盒
- CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))
- 别名
- Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2B 产品)
- 别名
- CAM2 ELISA Kit, CAMK2 ELISA Kit, CAMKB ELISA Kit, Camk2d ELISA Kit, Ck2b ELISA Kit, camk2 ELISA Kit, camk2b ELISA Kit, cam2 ELISA Kit, camkb ELISA Kit, CAMK2B ELISA Kit, calcium/calmodulin dependent protein kinase II beta ELISA Kit, calcium/calmodulin-dependent protein kinase II, beta ELISA Kit, calcium/calmodulin-dependent protein kinase II beta ELISA Kit, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog ELISA Kit, calcium/calmodulin dependent protein kinase (CaM kinase) II beta L homeolog ELISA Kit, calcium/calmodulin-dependent protein kinase (CaM kinase) II beta ELISA Kit, CAMK2B ELISA Kit, Camk2b ELISA Kit, camk2a.S ELISA Kit, camk2b.L ELISA Kit
- 背景
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Gene Name: Calcium/Calmodulin Dependent Protein Kinase II Beta
Gene Aliases: CAM2, CAMK2, CAMKB, Proline Rich Calmodulin-Dependent Protein Kinase
- 基因ID
- 816
- UniProt
- Q13554
- 途径
- WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
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