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C-Peptide ELISA 试剂盒

适用: 小鼠 Colorimetric Competition ELISA 98.77 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
产品编号 ABIN5652405
发货至: 中国
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    C-Peptide
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    检测范围
    98.77 pg/mL - 8000 pg/mL
    最低检测浓度
    98.77 pg/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of C-Peptide (CP). No significant cross-reactivity or interference between C-Peptide (CP) and analogues was observed.
    灵敏度
    37.35 pg/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    2 h
    板类型
    Pre-coated
    实验流程
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to C-Peptide (CP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled C-Peptide (CP) and unlabeled C-Peptide (CP) (Standards or samples) with the pre-coated antibody specific to C-Peptide (CP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of C-Peptide (CP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of C-Peptide (CP) in the sample.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Peptide (CP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Peptide (CP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all C-Peptide ELISA试剂盒
    C-Peptide
    Abstract
    C-Peptide 产品
    别名
    insulin 2 ELISA Kit, Ins2 ELISA Kit
    背景

    Gene Name: C-Peptide

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