RNF112 ELISA 试剂盒
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- 抗原 See all RNF112 ELISA试剂盒
- RNF112 (Ring Finger Protein 112 (RNF112))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Brain Finger Protein (BFP). No significant cross-reactivity or interference between Brain Finger Protein (BFP) and analogues was observed.
- 灵敏度
- 0.066 ng/mL
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Ring Finger Protein 112 (RNF112) ELISA Kit
RNF112 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ring Finger Protein 112 (RNF112) ELISA KitRNF112 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Finger Protein (BFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Finger Protein (BFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Finger Protein (BFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Finger Protein (BFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Finger Protein (BFP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Finger Protein (BFP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all RNF112 ELISA试剂盒
- RNF112 (Ring Finger Protein 112 (RNF112))
- 别名
- Brain Finger Protein (RNF112 产品)
- 别名
- BFP ELISA Kit, ZNF179 ELISA Kit, AI854096 ELISA Kit, R75574 ELISA Kit, Zfp179 ELISA Kit, bfp ELISA Kit, Bfb ELISA Kit, Znf179 ELISA Kit, rnf112 ELISA Kit, rnf112.S ELISA Kit, znf179 ELISA Kit, ring finger protein 112 ELISA Kit, ring finger protein 112 L homeolog ELISA Kit, RNF112 ELISA Kit, Rnf112 ELISA Kit, rnf112.L ELISA Kit
- 背景
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Gene Name: Brain Finger Protein
Gene Aliases: RNF179, RNF112, Ring Finger Protein 179, Ring Finger Protein 112
- 基因ID
- 22671
- UniProt
- Q96DY5
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