BMPR1A ELISA 试剂盒
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- 抗原 See all BMPR1A ELISA试剂盒
- BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.781 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.781 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein Receptor 1A (BMPR1A). No significant cross-reactivity or interference between Bone Morphogenetic Protein Receptor 1A (BMPR1A) and analogues was observed.
- 灵敏度
- 0.24 ng/mL
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein Receptor 1A (BMPR1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein Receptor 1A (BMPR1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein Receptor 1A (BMPR1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein Receptor 1A (BMPR1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1A (BMPR1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1A (BMPR1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all BMPR1A ELISA试剂盒
- BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))
- 别名
- Bone Morphogenetic Protein Receptor 1A (BMPR1A 产品)
- 别名
- 10q23del ELISA Kit, ACVRLK3 ELISA Kit, ALK3 ELISA Kit, CD292 ELISA Kit, SKR5 ELISA Kit, 1110037I22Rik ELISA Kit, AU045487 ELISA Kit, BMPR-IA ELISA Kit, Bmpr ELISA Kit, BMPR-1 ELISA Kit, BRK-1 ELISA Kit, BMPR1A ELISA Kit, acvrlk3 ELISA Kit, alk-3 ELISA Kit, alk3 ELISA Kit, cd292 ELISA Kit, skr5 ELISA Kit, BR1a ELISA Kit, alk3tr ELISA Kit, bmpr1a ELISA Kit, zgc:136731 ELISA Kit, wu:fe05b06 ELISA Kit, wu:fe06e10 ELISA Kit, zgc:92227 ELISA Kit, bone morphogenetic protein receptor type 1A ELISA Kit, bone morphogenetic protein receptor, type 1A ELISA Kit, bone morphogenetic protein receptor type 1A S homeolog ELISA Kit, bone morphogenetic protein receptor, type IAa ELISA Kit, bone morphogenetic protein receptor, type IAb ELISA Kit, BMPR1A ELISA Kit, Bmpr1a ELISA Kit, bmpr1a ELISA Kit, bmpr1a.S ELISA Kit, bmpr1aa ELISA Kit, bmpr1ab ELISA Kit
- 背景
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Gene Name: Bone Morphogenetic Protein Receptor 1A
Gene Aliases: CD292, ACVRLK3, ALK3, SKR5, BMPR1-A, Activin A Receptor, Type Ii-Like Kinase 3, Activin Receptor-Like Kinase 3, Serine/Threonine-Protein Kinase Receptor R5
- 基因ID
- 81507
- UniProt
- Q78EA7
- 途径
- Stem Cell Maintenance
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