BLMH ELISA 试剂盒
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- 抗原 See all BLMH ELISA试剂盒
- BLMH (Bleomycin Hydrolase (BLMH))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Bleomycin Hydrolase (BLMH). No significant cross-reactivity or interference between Bleomycin Hydrolase (BLMH) and analogues was observed.
- 灵敏度
- 0.055 ng/mL
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Bleomycin Hydrolase (BLMH) ELISA Kit
BLMH 适用: 人 Colorimetric Sandwich ELISA 28 pg/mL - 1800 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Bleomycin Hydrolase (BLMH) ELISA KitBLMH 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bleomycin Hydrolase (BLMH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bleomycin Hydrolase (BLMH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bleomycin Hydrolase (BLMH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bleomycin Hydrolase (BLMH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bleomycin Hydrolase (BLMH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bleomycin Hydrolase (BLMH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all BLMH ELISA试剂盒
- BLMH (Bleomycin Hydrolase (BLMH))
- 别名
- Bleomycin Hydrolase (BLMH 产品)
- 别名
- AI035728 ELISA Kit, Bh ELISA Kit, Bmh ELISA Kit, wu:fb13c01 ELISA Kit, zgc:66261 ELISA Kit, bh ELISA Kit, bmh ELISA Kit, BH ELISA Kit, BMH ELISA Kit, bleomycin hydrolase ELISA Kit, Bleomycin hydrolase ELISA Kit, bleomycin hydrolase S homeolog ELISA Kit, BLMH ELISA Kit, pepC ELISA Kit, LOC5569945 ELISA Kit, Apre_0140 ELISA Kit, Apar_0899 ELISA Kit, Smon_1289 ELISA Kit, Kfla_1242 ELISA Kit, Snas_3912 ELISA Kit, BLJ_1941 ELISA Kit, Olsu_1085 ELISA Kit, PGTDC60_0106 ELISA Kit, Blmh ELISA Kit, blmh ELISA Kit, blmh.S ELISA Kit
- 背景
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Gene Name: Bleomycin Hydrolase
Gene Aliases: BH, BMH
- 基因ID
- 642
- UniProt
- Q13867
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