Biglycan ELISA 试剂盒
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- 抗原 See all Biglycan (BGN) ELISA试剂盒
- Biglycan (BGN)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Biglycan (BGN). No significant cross-reactivity or interference between Biglycan (BGN) and analogues was observed.
- 灵敏度
- 0.127 ng/mL
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Biglycan (BGN) ELISA Kit
BGN 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Biglycan (BGN) ELISA KitBGN 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Biglycan (BGN) ELISA KitBGN 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Biglycan (BGN) ELISA KitBGN 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Biglycan (BGN) ELISA KitBGN 适用: 小鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Biglycan (BGN) ELISA KitBGN 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Biglycan (BGN) ELISA KitBGN 适用: 人 AA 38-368 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biglycan (BGN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Biglycan (BGN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Biglycan (BGN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biglycan (BGN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Biglycan (BGN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Biglycan (BGN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Biglycan (BGN) ELISA试剂盒
- Biglycan (BGN)
- 别名
- Biglycan (BGN 产品)
- 别名
- DSPG1 ELISA Kit, PG-S1 ELISA Kit, PGI ELISA Kit, SLRR1A ELISA Kit, BG ELISA Kit, BSPG1 ELISA Kit, bgn ELISA Kit, zgc:91989 ELISA Kit, dspg1 ELISA Kit, pg-s1 ELISA Kit, slrr1a ELISA Kit, xbgn ELISA Kit, BGN ELISA Kit, zgc:77123 ELISA Kit, DKFZp468I2427 ELISA Kit, biglycan ELISA Kit, biglycan L homeolog ELISA Kit, biglycan a ELISA Kit, biglycan S homeolog ELISA Kit, biglycan b ELISA Kit, BGN ELISA Kit, Bgn ELISA Kit, bgn.L ELISA Kit, bgna ELISA Kit, bgn.S ELISA Kit, bgn ELISA Kit, bgnb ELISA Kit
- 背景
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Gene Name: Biglycan
Gene Aliases: DSPG1, PG-S1, PGI, SLRR1A, Biglycan Proteoglycan, Bone/cartilage proteoglycan I
- 基因ID
- 633
- UniProt
- P21810
- 途径
- Glycosaminoglycan Metabolic Process
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