Aspartate beta Hydroxylase ELISA 试剂盒
-
- 抗原 See all Aspartate beta Hydroxylase (ASPH) ELISA试剂盒
- Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Aspartate Beta Hydroxylase (ASPH). No significant cross-reactivity or interference between Aspartate Beta Hydroxylase (ASPH) and analogues was observed.
- 灵敏度
- 0.057 ng/mL
- Featured
- Discover our best selling ASPH ELISA Kit
- Top Product
- Discover our top product ASPH ELISA Kit
-
Aspartate beta-Hydroxylase (ASPH) ELISA Kit
ASPH 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Beta Hydroxylase (ASPH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aspartate Beta Hydroxylase (ASPH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Beta Hydroxylase (ASPH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aspartate Beta Hydroxylase (ASPH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aspartate Beta Hydroxylase (ASPH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aspartate Beta Hydroxylase (ASPH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all Aspartate beta Hydroxylase (ASPH) ELISA试剂盒
- Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))
- 别名
- Aspartate Beta Hydroxylase (ASPH 产品)
- 别名
- AAH ELISA Kit, BAH ELISA Kit, CASQ2BP1 ELISA Kit, HAAH ELISA Kit, JCTN ELISA Kit, junctin ELISA Kit, 2310005F16Rik ELISA Kit, 3110001L23Rik ELISA Kit, AI848629 ELISA Kit, AW261690 ELISA Kit, AW561901 ELISA Kit, C79816 ELISA Kit, cI-37 ELISA Kit, aah ELISA Kit, bah ELISA Kit, jctn ELISA Kit, MGC107896 ELISA Kit, cb971 ELISA Kit, fb69e10 ELISA Kit, fc06d04 ELISA Kit, fc95a08 ELISA Kit, wu:fb69e10 ELISA Kit, wu:fc06d04 ELISA Kit, wu:fc95a08 ELISA Kit, aspartate beta-hydroxylase ELISA Kit, aspartate-beta-hydroxylase ELISA Kit, aspartate beta-hydroxylase L homeolog ELISA Kit, ASPH ELISA Kit, Asph ELISA Kit, asph ELISA Kit, asph.L ELISA Kit
- 背景
-
Gene Name: Aspartate Beta Hydroxylase
Gene Aliases: BAH, CASQ2BP1, HAAH, JCTN, Junctin, Humbug, Junctate, Aspartyl/Asparaginyl Beta-Hydroxylase, Peptide-aspartate beta-dioxygenase
- 基因ID
- 444
- UniProt
- Q12797
- 途径
- Positive Regulation of Endopeptidase Activity
-
