APEX1 ELISA 试剂盒
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- 抗原 See all APEX1 ELISA试剂盒
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Apurinic/Apyrimidinic Endonuclease 1 (APEX1). No significant cross-reactivity or interference between Apurinic/Apyrimidinic Endonuclease 1 (APEX1) and analogues was observed.
- 灵敏度
- 0.058 ng/mL
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Apurinic/Apyrimidinic Endonuclease 1 (APEX1) ELISA Kit
APEX1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) ELISA KitAPEX1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) ELISA KitAPEX1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apurinic/Apyrimidinic Endonuclease 1 (APEX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apurinic/Apyrimidinic Endonuclease 1 (APEX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apurinic/Apyrimidinic Endonuclease 1 (APEX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apurinic/Apyrimidinic Endonuclease 1 (APEX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apurinic/Apyrimidinic Endonuclease 1 (APEX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apurinic/Apyrimidinic Endonuclease 1 (APEX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all APEX1 ELISA试剂盒
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
- 别名
- Apurinic/Apyrimidinic Endonuclease 1 (APEX1 产品)
- 别名
- apex1 ELISA Kit, MGC76280 ELISA Kit, MGC79645 ELISA Kit, MGC78928 ELISA Kit, APE ELISA Kit, APE1 ELISA Kit, APEN ELISA Kit, APEX ELISA Kit, APX ELISA Kit, HAP1 ELISA Kit, REF1 ELISA Kit, Apex ELISA Kit, REF-1 ELISA Kit, BAP1 ELISA Kit, apex1a ELISA Kit, hm:zehn1532 ELISA Kit, wu:fc13d11 ELISA Kit, zgc:66204 ELISA Kit, Ref-1 ELISA Kit, apurinic/apyrimidinic endodeoxyribonuclease 1 ELISA Kit, APEX nuclease (multifunctional DNA repair enzyme) 1 ELISA Kit, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog ELISA Kit, DNA-(apurinic or apyrimidinic site) lyase ELISA Kit, apurinic/apyrimidinic endonuclease 1 ELISA Kit, APEX1 ELISA Kit, apex1 ELISA Kit, apex1.L ELISA Kit, CNG03640 ELISA Kit, CNH01280 ELISA Kit, nth ELISA Kit, Spirs_4119 ELISA Kit, Palpr_0103 ELISA Kit, Apex1 ELISA Kit
- 背景
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Gene Name: Apurinic/Apyrimidinic Endonuclease 1
Gene Aliases: APE, APE1, APEN, APEX, APX, HAP1, REF1, Multifunctional DNA Repair Enzyme, APEX Nuclease 1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase
- 途径
- DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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