Apelin ELISA 试剂盒
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- 抗原 See all Apelin (APLN) ELISA试剂盒
- Apelin (APLN)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 98.77 pg/mL - 8000 pg/mL
- 最低检测浓度
- 98.77 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Apelin (APLN). No significant cross-reactivity or interference between Apelin (APLN) and analogues was observed.
- 灵敏度
- 35.3 pg/mL
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Apelin (APLN) ELISA Kit
APLN 适用: 人 Colorimetric Competition ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Apelin (APLN) ELISA KitAPLN 适用: 人 Colorimetric Competition ELISA 19.75 pg/mL - 1600 pg/mL Plasma, Serum
Apelin (APLN) ELISA KitAPLN 适用: 小鼠 Colorimetric Competition ELISA 98.77 pg/mL - 8000 pg/mL Plasma, Serum
Apelin (APLN) ELISA KitAPLN 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apelin (APLN) ELISA KitAPLN 适用: 人 Colorimetric Competition ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Apelin (APLN) ELISA KitAPLN 适用: 小鼠 Colorimetric Competition ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Apelin (APLN) ELISA KitAPLN 适用: 绵羊 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Apelin (APLN) ELISA KitAPLN 适用: Pig Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Apelin (APLN) ELISA KitAPLN 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apelin (APLN) ELISA KitAPLN 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin (APLN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin (APLN) and unlabeled Apelin (APLN) (Standards or samples) with the pre-coated antibody specific to Apelin (APLN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin (APLN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apelin (APLN) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin (APLN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apelin (APLN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Apelin (APLN) ELISA试剂盒
- Apelin (APLN)
- 别名
- Apelin (APLN 产品)
- 别名
- APLN ELISA Kit, Xapelin ELISA Kit, xnpep2 ELISA Kit, preproapelin ELISA Kit, 6030430G11Rik ELISA Kit, Apel ELISA Kit, APEL ELISA Kit, XNPEP2 ELISA Kit, apelin ELISA Kit, apelin S homeolog ELISA Kit, apln ELISA Kit, APLN ELISA Kit, apln.S ELISA Kit, Apln ELISA Kit
- 背景
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Gene Name: Apelin
Gene Aliases: XNPEP2, APEL, APJ endogenous ligand
- 基因ID
- 30878
- UniProt
- Q9R0R4
- 途径
- Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Feeding Behaviour
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