Annexin IV ELISA 试剂盒
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- 抗原 See all Annexin IV (ANXA4) ELISA试剂盒
- Annexin IV (ANXA4) (Annexin A4 (ANXA4))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 18.75 pg/mL - 1200 pg/mL
- 最低检测浓度
- 18.75 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Annexin A4 (ANXA4). No significant cross-reactivity or interference between Annexin A4 (ANXA4) and analogues was observed.
- 灵敏度
- 7.4 pg/mL
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Annexin A4 (ANXA4) ELISA Kit
ANXA4 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Annexin A4 (ANXA4) ELISA KitANXA4 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A4 (ANXA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A4 (ANXA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A4 (ANXA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A4 (ANXA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A4 (ANXA4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A4 (ANXA4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Annexin IV (ANXA4) ELISA试剂盒
- Annexin IV (ANXA4) (Annexin A4 (ANXA4))
- 别名
- Annexin A4 (ANXA4 产品)
- 别名
- ANXA4 ELISA Kit, Anx4 ELISA Kit, XAnx4 ELISA Kit, pig28 ELISA Kit, X-anx4 ELISA Kit, Xanx-4 ELISA Kit, ANXIV ELISA Kit, anx4 ELISA Kit, ANX4 ELISA Kit, PIG28 ELISA Kit, ZAP36 ELISA Kit, Annexin-4 ELISA Kit, P32.5 ELISA Kit, PAP-II ELISA Kit, PP4-X ELISA Kit, AI265406 ELISA Kit, AIV ELISA Kit, AW106930 ELISA Kit, annexin A4 ELISA Kit, annexin A4 L homeolog ELISA Kit, ANXA4 ELISA Kit, anxa4 ELISA Kit, CC1G_00713 ELISA Kit, LOC100280951 ELISA Kit, Anxa4 ELISA Kit, anxa4.L ELISA Kit
- 背景
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Gene Name: Annexin A4
Gene Aliases: ANX-A4, ANX4, PIG28, 35-beta calcimedin, Carbohydrate-binding protein p33/p41, Chromobindin-4, Endonexin I, Lipocortin IV, Placental anticoagulant protein II
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