PLAP ELISA 试剂盒
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- 抗原 See all PLAP (ALPP) ELISA试剂盒
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 125.00 pg/mL - 8000 pg/mL
- 最低检测浓度
- 125.00 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Placental (ALPP). No significant cross-reactivity or interference between Alkaline Phosphatase, Placental (ALPP) and analogues was observed.
- 灵敏度
- 52 pg/mL
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Placental Alkaline Phosphatase (ALPP) ELISA Kit
ALPP 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Placental Alkaline Phosphatase (ALPP) ELISA KitALPP 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Placental Alkaline Phosphatase (ALPP) ELISA KitALPP 适用: 人 Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Placental (ALPP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Placental (ALPP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Placental (ALPP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Placental (ALPP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase, Placental (ALPP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase, Placental (ALPP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PLAP (ALPP) ELISA试剂盒
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
- 别名
- Alkaline Phosphatase, Placental (ALPP 产品)
- 别名
- ECK0378 ELISA Kit, JW0374 ELISA Kit, psiA ELISA Kit, ALP ELISA Kit, PALP ELISA Kit, PLAP ELISA Kit, PLAP-1 ELISA Kit, ILC ELISA Kit, CTACK ELISA Kit, ESkine ELISA Kit, Akp2 ELISA Kit, alp ELISA Kit, zgc:56672 ELISA Kit, DDBDRAFT_0205491 ELISA Kit, DDBDRAFT_0231570 ELISA Kit, DDB_0205491 ELISA Kit, DDB_0231570 ELISA Kit, ALPI ELISA Kit, Actn2lp ELISA Kit, Alp ELISA Kit, bacterial alkaline phosphatase ELISA Kit, alkaline phosphatase, placental ELISA Kit, alkaline phosphatase, placental type ELISA Kit, C-C motif chemokine ligand 27 ELISA Kit, alkaline phosphatase, liver/bone/kidney ELISA Kit, alkaline phosphatase ELISA Kit, alkaline phosphatase, intestinal ELISA Kit, PDZ and LIM domain 3 ELISA Kit, phoA ELISA Kit, ALPP ELISA Kit, LOC100436725 ELISA Kit, CCL27 ELISA Kit, alpl ELISA Kit, alp ELISA Kit, ALPI ELISA Kit, Pdlim3 ELISA Kit, Alpp ELISA Kit
- 背景
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Gene Name: Alkaline Phosphatase, Placental
Gene Aliases: PLAP1, ALP, ALPG, ALPPL, GCAP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1
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